Choices

Additional Delicate than MTT, MTS, or WST-1

No Toxicity to Cell

Easier Steps, No pure solvents required

Frequent One Bottle Reply, ready-to-use

Background

Cell proliferation will possibly be detected by fairly just a few methods. Cell proliferation induces an increase all by means of the follow of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt to formazan.

The WST Cell Proliferation Colorimetric Assay Bundle deal plus offers among the many best and most delicate method for quantification of cell proliferation and viability. Cell proliferation causes the rise all by means of the amount of formazan dye common that is straight proportional to the number of residing cells and might possibly be quantified by measuring the absorbance of the dye decision at 440 nm using microtiter plate reader. The gear may be utilized for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer remedy and loads of fully completely completely different toxic brokers.

The assay may be used for the detection of cell proliferation in response to cytokines, mitogens, progress parts and dietary dietary nutritional vitamins, and so forth. The assay would possibly very properly be very simple and simple, merely add-and-read, requiring no solubilization, no washing and no harvesting steps, and is widespread, sooner and additional delicate than MTS, MTT or XTT-based assays. Your full assay will possibly be carried out in a microtiter plate and correlates accurately with the [3H]-thymidine incorporation assay.

Description

Our product Cell Counting Bundle deal-8 (CCK-8) current a further helpful and delicate technique for the evaluation of cell amount dedication and cell proliferationtotoxicity assay than earlier strategies. The gear makes use of a really water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon low value all by means of the presence of an electron mediator. The amount of the formazan generated by dehydrogenases is straight in proportion to the numbers of residing cells. The detection sensitivity by CCK-Eight is bigger than fully completely completely different tetrazolium salts resembling MTT, XTT, MTS or WST-1.

Cell Counting Kit-8 Apexbio

Cell Counting Equipment-Eight Apexbio

Choices & Properties

Choices Fast and helpful Straightforward one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The method could be very straightforward that requires no washing/harvesting/or solubilization steps and is faster and additional delicate than MTT/XTT/or MTS-based assays. Your full assay will possibly be carried out straight in a 96-well plate.
Present Gel pack
Storage Circumstances Retailer at -20°C.
Apexbio Cell Counting Kit-8

Apexbio Cell Counting Equipment-8

 

 

CCK-8 Cell Counting Kit

A311-02 1000 rxn
EUR 234

Cell Counting Kit-8 (CCK-8)

AR1160 5 mL (for 500 assays, 10μL per well)
EUR 131

Cell Counting Kit-8 (CCK-8)

B34302 5 mL (500 ractions)
EUR 130
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK-8)

B34304 25 mL (2500 ractions)
EUR 315
Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading.

Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit

abx090677-500tests 500 tests
EUR 394

Bacterial Counting Colorimetric Assay Kit

K511-2500
EUR 620

Bacterial Counting Colorimetric Assay Kit

K511-500
EUR 349

CORNING COUNTING CHAMBER FOR CORNING CELL COUNTER

480200 1/pk
EUR 142
Description: Lab Equipment; Cell Counters

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT 1 Kit
EUR 2798

CytoSelect BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 531
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT 1 Kit
EUR 2798

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

HIF-1 Alpha Cell Based ELISA Kit

CBA-281 96 assays
EUR 612
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.

CytoSelect 48-Well Cell Contraction Assay Kit

CBA-5021 48 assays
EUR 635
Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction.

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT 1 Kit
EUR 4335

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT 1 Kit
EUR 4335

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132