Form: lyophilized powder
Quality level: 200
Storage temperature: 2-8°C
Product Name: Bordetella pertussis Recombinants
Bordetella pertussis Tohama I … ptxA(2665068) , ptxB(2665069) , ptxC(2665408)
The product is not activated. If activation is required, see Kaslow, et al., for suggested conditions.
The toxin is released from B. pertussis in an inactive form. When pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer is activated, perhaps through the action of glutathione and ATP. Kaslow, et al. provide a protocol for activating pertussis toxin in vitro.
Pertussis toxin catalyzes the ADP-ribosylation of the α-subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and GT. This prevents G-protein heterotrimers from interacting with the receptors, thus blocking their docking and activation. Since the Gα subunits remain in their inactive GDP-bound state, they cannot inactivate adenylyl cyclase or open K+ channels.
Lyophilized powder containing buffer salts of sodium chloride and sodium phosphate