Concanavalin A – from Canavalia ensiformis

Published by Derek Porter on

DESCRIPTION Utility

Concanavalin A from Canavalia ensiformis (Jack bean) has been used for creating zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been moreover used all by means of the preparation of protein loaded microgels to cease enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Packaging

1 g in poly bottle
25, 100, 250 mg in poly bottle

PRODUCT DESCRIPTION

Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are typically found all by means of the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.

  • Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
  • Detect glycoconjugates in microscopy and circulation cytometry
  • Stain glycoproteins in gels
  • Withstands fixation and permeabilization
  • Number of 10 CF dyes from UV to near-infrared
  • Superior CF dyes are good, photostable, and water-soluble

Lectins are moreover versatile probes for detecting glycoconjugates in microscopy and circulation cytometric options and for gel staining of glycoproteins. In neutral and alkaline picks, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic picks (pH beneath 5.0), Con A exists as a dimer. Con A is prone to be utilized to selectively stain the cell ground of dwell cells, and stand as lots as fixation and permeabilization. When cells are mounted and permeabilized forward of staining, fluorescent lectins stain every cell ground and organelles all by means of the secretory pathway.

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Biochem/physiol Actions

Con A should not be blood group particular nonetheless has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for put together. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero bigger aggregates are long-established. Con A reveals mitogenic put together which relies upon its diploma of aggregation. Succinylation results in an brisk dimeric type which stays a dimer above pH 5.6.

Analysis Uncover

Agglutination put together: Determined from serial dilutions of a 1 mg/mL reply (this put together is the underside conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Sort A Human Blood.
Concanavalin A (Con A) may be going probably most likely probably the most typically used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A reveals the acute, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.

Uncover the Proper Stain for Your Utility

Con A and totally completely totally different lectins are carbohydrate binding proteins that acknowledge particular sugar moieties on glycoproteins.  The presence and distribution of these targets fluctuate between cell varieties and tissues. In consequence, totally completely totally different cell ground stains or totally completely totally different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, would possibly produce greater ground staining and will also be further acceptable in your cell type. Lectin conjugates is prone to be utilized to selectively stain the cell ground of dwell cells, and stand as lots as fixation and permeabilization.

Concanavalin A (ConA)
05-0106-100 100 mg
EUR 76

Concanavalin A (ConA)
05-0106-1000 1 g
EUR 199

Concanavalin A (ConA)
05-0106-10000 10 g Ask for price

Concanavalin A (ConA)
05-0106-100000 100 g Ask for price

Concanavalin A (ConA)
05-0106-250 250 mg
EUR 88

Concanavalin A (Con A) Antibody
20-abx101152
  • EUR 272.00
  • EUR 133.00
  • EUR 676.00
  • EUR 342.00
  • EUR 244.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Concanavalin A ELISA Kit
20-abx052181
  • EUR 6439.00
  • EUR 3432.00
  • EUR 801.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Concanavalin A, CF350 conjugate
29015 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF488A conjugate
29016 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF594 conjugate
29017 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF633 conjugate
29018 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF640R conjugate
29019 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF680 conjugate
29020 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF770 conjugate
29058 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF405M conjugate
29074 5x1mg
EUR 219
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF405S conjugate
29075 5x1mg
EUR 219
Description: Minimum order quantity: 1 unit of 5x1mg

Concanavalin A, CF750 conjugate
29080 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg

Goat Concanavalin A ELISA kit
E06C0795-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Concanavalin A ELISA kit
E06C0795-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Concanavalin A ELISA kit
E06C0795-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Concanavalin A ELISA kit
E02C0795-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Concanavalin A ELISA kit
E02C0795-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Concanavalin A ELISA kit
E02C0795-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Concanavalin A ELISA kit
E03C0795-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Concanavalin A ELISA kit
E03C0795-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Concanavalin A ELISA kit
E03C0795-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Concanavalin A ELISA kit
E04C0795-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Concanavalin A ELISA kit
E04C0795-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Concanavalin A ELISA kit
E04C0795-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Concanavalin A in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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