Benefits

  • Speedy amplification: The amplification cost of the polymerase is 6kb/min. 1kb fragment may very well be amplified inside 25min.
  • Extended fragment amplification: For plasmid, λ DNA and fully totally different simple templates, the polymerase can effectively amplify > 20kb. For genome, the polymerase can effectively amplify > 8kb. And for cDNA, the polymerase can effectively amplify > 8kb.
  • Excessive specificity: With scorching begin expertise, the polymerase is 100% inactive beneath 50°C, and will solely be restored by heating at 95°C for 5min.
  • Excessive tolerance to impurities: Samples of entire blood, serum, cultured cells and urine may very well be immediately amplified with out prior DNA purification.

What’s Direct PCR?

Direct PCR (dPCR) is a technique of DNA amplification immediately from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This method significantly reduces experimental time, and value in genotyping and high-volume duties. It furthermore gives a bigger choice when confronted with the challenges of amplifying a extraordinarily small amount of pattern the place the purification step might doubtlessly result in pattern loss.

In some strategies, dPCR works like typical PCR. Each strategies rely upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The first distinction between dPCR and customary PCR is the tailor-made buffers utilized in dPCR. The modified parts of dPCR allow sturdy amplification regardless of the presence of PCR inhibitors often present in crude samples.

ALS SARS-CoV-2 RT-PCR 4G has been developed and produced totally at ALS in Portugal and is designed primarily for export. This new gear is ready to detect all acknowledged SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with fully totally different acknowledged associated viruses or frequent respiratory pathogens. The gathering of the genomic areas used as targets and the sequences of the primers and hydrolysis probes have been primarily based completely on WHO, CDC and DGS options.

Direct PCR Reagent

Direct PCR Reagent

The gear makes use of the RT-PCR method, combining amplification of sure areas of the viral genome with its detection by specific hydrolysis probes. The SARS-CoV-2 genome goal areas are terribly conserved and correspond to 2 specific genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The gear also can detect the gene that codifies the structural protein of the envelope (gene E) specific to the Sarbecovirus subgenus.

Unit Definition

One unit is printed as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.

Description Code Specification Class Know-how
ALS SARS-CoV-2 RT-PCR Try for surfaces ALS-B01-Adv RUO Meals /Water Virology Detection Exact-Time PCR
ALS SARS-CoV-2 RT-PCR Try for Wastewater ALS-B02-Adv RUO Meals /Water Virology Detection Exact-Time PCR

 

Direct PCR Complete Kit

S700 100 rcs
EUR 133

Direct PCR Complete Kit

S700-2 5x100 rcs
EUR 524

amfiSure Direct PCR Lysis Buffer

D0300-010 100ml
EUR 138

Blood Direct PCR Kit V2

PD103-01 50 rxn
EUR 169

Blood Direct PCR Kit V2

PD103-02 200 rxn
EUR 354

Mouse Direct PCR Kit (For Genotyping)

B40013 200 ractions
EUR 209
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B40015 500 ractions
EUR 409
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45012 1ml*5 (500 ractions)
EUR 280
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45013 1ml*20 (2000 ractions)
EUR 865
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

FastAmp Plant Direct PCR Kit - 250 Reactions

4612 1/EA
EUR 164

FastAmp Plant Direct PCR Kit - 1250 Reactions

4615 1/EA
EUR 518

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-100P 100 Preps Ask for price

Direct RT-PCR Kit wioRealStart DNA Polymerase

FYT507-50P 50 Preps Ask for price

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02100 100 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Human Blood Direct PCR Kit

ABP-PP-BD02500 500 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02100 100 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Allele-In-One Mouse Tail Direct PCR Kit

ABP-PP-MT02500 500 RXNS Ask for price
    • Product line: One-step Genotyping
    • Brand:

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-100P 100 Preps Ask for price

Direct RT-PCR Kit wi RealStart DNA Polymerase

FYT506-50P 50 Preps; Ask for price

MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS

MULTIPLEX KIT PCR MASTITIS PCR kit

PCR-MPX218-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS

MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

MULTIPLEX KIT PCR Babesia & Theileria PCR kit

PCR-MPX401-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR Babesia & Theileria

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-540 20 rcs x 50 µl
EUR 164

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-542 100 rcs x 50 µl
EUR 549

One.Direct.Step RT-qPCR Kit for Probes (DIRECT PCR FROM BLOOD)

105-544 1000 rcs x 50 µl
EUR 4189

Halobacillus PCR kit

PCR-A635-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Halobacillus

Halobacillus PCR kit

PCR-A635-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Halobacillus

Enterovirus PCR kit

PCR-H433-48R 50T
EUR 524.4
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Enterovirus