Exosome Isolation Kits

A high-efficient exosome isolation kit can help a lot for every exosome researchers. High purity, high yield, and easy operation make it a great research tool.

As we know, exosomes are small membrane vesicles (30–150 nm) of endocytic origin, which are shed by all cell types under normal- and patho-physiological conditions. Exosomes have pleiotropic physiological and pathological functions and play significant roles in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Exosomes have received significant attention for their roles in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation such as differential ultracentrifugation, density gradient ultracentrifugation, ultracentrifugation, chromatography, magnetic bead immunoassay, polypolymer precipitation method, etc. However, the quick and easy operation of exosome isolation kits is irreplaceable.

CUSABIO Exosome Isolation Kits purify exosome by affinity purification. High Purity exosomes and other Evs (Extracellular vesicles) from cell culture medium and body fluid (high yield by normal microfiltration) can be easily obtained by this method.

 

Application

Isolate pure exosome from cell culture media (supernatant). This kit yields much higher purity than exosome precipitation method. This product is for research use only.

Yield

Each reaction can process 2 ~ 4 ml cell culture medium. The yield of each reaction can yield pure exosome suspended in 50 ~ 200 µl PBS .

Description

P100 kit is for exosome purification / isolation at high yield and purity from cell culture media.

  • Easy to use: No ultra-centrifugation (< 2 hours)
  • 10 fold higher yield (vs. other kits and ultracentrifuge)
  • Save cost (vs. antibodies-beads method)
  • Isolate Pure exosome (>95% purity vs. 25% purity by exosome precipitation method)
  • Intact exosome (good morphology)

Use as little as 2 ml cell media to achieve high yield of exosomes for any downstream applications: EM study, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins.

Shipping / Storage

Ship and store at room temperature. Upon receiving keep all bottles upright, in cool and dark place.

Shelf Life

12 months
PureExo Exosome Isolation kit

PureExo Exosome Isolation kit

Abstract

Exosomes have received significant attention for their role in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation. Here we compared the efficacy of four kits from Invitrogen, 101Bio, Wako and iZON along with conventional ultracentrifugation-based method for exosome yield, purity and quality. Cell culture supernatant was used as an abundant source of exosomes, and exosome quantity, size-distribution, zeta-potential, marker-expression and RNA/protein quality were determined.

The Invitrogen kit gave the highest yield but the preparation showed broader size-distribution likely due to microvesicle co-precipitation and had the least dispersion stability. Other preparations showed <150 nm size range and good stability. Preparation from iZON column; however, had a broader size-distribution in the lower size range suggestive of some impurities of non-vesicular aggregates.

RNA quality from all preparations was comparable; however, proteins from Invitrogen method-based exosomal preparation showed polyethylene glycol (PEG) contamination in mass spectrometry. Chemical impurities from the precipitant could also be the cause of toxicity of Invitrogen method-based exosomal preparation in biological growth measurement assay. Together, these findings should serve as a guide to choose and further optimize exosome isolation methods for their desired downstream applications.

  • Suitable for many downstream experiments

    The experiments include Transmission electron microscope analysis, Nanoparticle tracking analysis, NanoFCM analysis, Western Blot, Fluorescence quantitative (qPCR), High-Throughput Sequencing, etc.

  • Suitable for a variety of sample types

    CUSABIO Exosome isolation kit provides a simple and reliable method to extract intact exosomes from the cell culture supernatant, serum, plasma, latex, urine, saliva.

 

Exosome Isolation kit (for serum & plasma)
P101 NULL
EUR 0
Exosome Isolation kit (for serum & plasma)
P101L NULL
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Exosome Isolation kit (for serum & plasma)
P101S NULL
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Exosome Isolation kit (forHuman Body Fluid)
P121 NULL
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Exosome Isolation kit (forHuman Body Fluid)
P121L NULL
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Exosome Isolation kit (forHuman Body Fluid)
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Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)
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Exosome Isolation kit (for cell culture media)
P100 NULL
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Exosome Isolation kit (for cell culture media)
P100L NULL
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Exosome Isolation kit (for cell culture media)
P100S NULL
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ExoPure? Reagent (Overall Exosome Isolation, biological fluids)
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ExoPure? Reagent (Overall Exosome Isolation, biological fluids)
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ExoPure? Reagent (Overall Exosome Isolation, cell media)
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