Precision
Intra-assay
| Pattern |
n |
Degree out |
SD |
CV% |
| 1 |
16 |
147pg/ml |
10.29 |
= 7% |
| 2 |
16 |
683pg/ml |
49.85 |
= 7.3% |
| 3 |
16 |
2750pg/ml |
206.25 |
= 7.5% |
Inter-assay
| Pattern |
n |
Degree out |
SD |
CV% |
| 1 |
24 |
156pg/ml |
13.88 |
= 8.9% |
| 2 |
24 |
700pg/ml |
62.3 |
= 8.9% |
| 3 |
24 |
2531pg/ml |
227.05 |
= 8.9% |
Product overview
The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom-made supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA gear relies upon on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for frequent: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Delicate is added and unbound conjugates are washed away with PBS or TBS buffer.
Key alternatives and particulars
- Sensitivity: 10 pg/ml
- Differ: 78 pg/ml – 5000 pg/ml
- Pattern sort: Cell custom-made supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection approach: Colorimetric
- Assay sort: Sandwich (quantitative)
- Reacts with: Human
-
Product prepare
Human BAFF-R ELISA Gadgets
-
Detection approach
Colorimetric
-
Pattern sort
Cell custom-made supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
-
Assay sort
Sandwich (quantitative)
-
Sensitivity
< 10 pg/ml
-
Differ
78 pg/ml – 5000 pg/ml
-
Assay time
3h 30m
-
Assay measurement
A substantial amount of steps commonplace assay
-
Species reactivity
Reacts with: Human
bio human baff r
-
Notes
BAFF-R (moreover usually known as Tumor necrosis draw back receptor superfamily member 13C or B-cell-activating draw back receptor) is a protein that in of us is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating draw back (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a sort III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
-
Platform
Pre-coated microplate (12 x Eight appropriately strips)
 BIO-X-ACT Short DNA Polymerase |
|
BIO-21065 |
Bioline |
500 Units |
Ask for price |
 BIO-X-ACT Short Mix, 2x |
|
BIO-25026 |
Bioline |
500 Reactions |
Ask for price |
 BAFF-R Polyclonal Antibody |
|
3578-100 |
Biovision |
|
EUR 316 |
 BAFF-R Blocking Peptide |
|
3578RBP-50 |
Biovision |
|
EUR 153 |
 Polyclonal BAFF-R Antibody |
|
APR15125G |
Leading Biology |
0.1mg |
EUR 484 |
|
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications: |
 Monoclonal Antibody (11C1)) Anti-BAFF-R (human) Monoclonal Antibody (11C1) |
|
M02865 |
BosterBio |
100ug |
EUR 446 |
|
Description: Mouse Monoclonal BAFF-R (human) Antibody (11C1). Validated in Flow Cytometry, IHC and tested in Human. |
 ELISA Kit) Human B-Cell Activating Factor (BAFF) ELISA Kit |
|
DLR-BAFF-Hu-48T |
DL Develop |
48T |
EUR 498 |
- Should the Human B-Cell Activating Factor (BAFF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Human B-Cell Activating Factor (BAFF) ELISA Kit |
|
DLR-BAFF-Hu-96T |
DL Develop |
96T |
EUR 647 |
- Should the Human B-Cell Activating Factor (BAFF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
|
|
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
