Precision

Intra-assay
Pattern n Degree out SD CV%
1 16 147pg/ml 10.29 = 7%
2 16 683pg/ml 49.85 = 7.3%
3 16 2750pg/ml 206.25 = 7.5%
Inter-assay
Pattern n Degree out SD CV%
1 24 156pg/ml 13.88 = 8.9%
2 24 700pg/ml 62.3 = 8.9%
3 24 2531pg/ml 227.05 = 8.9%

Product overview

The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom-made supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA gear relies upon on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for frequent: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Delicate is added and unbound conjugates are washed away with PBS or TBS buffer.

Key alternatives and particulars

  • Sensitivity: 10 pg/ml
  • Differ: 78 pg/ml – 5000 pg/ml
  • Pattern sort: Cell custom-made supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection approach: Colorimetric
  • Assay sort: Sandwich (quantitative)
  • Reacts with: Human
  • Product prepare

    Human BAFF-R ELISA Gadgets
  • Detection approach

    Colorimetric
  • Pattern sort

    Cell custom-made supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay sort

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Differ

    78 pg/ml – 5000 pg/ml
  • Assay time

    3h 30m
  • Assay measurement

    A substantial amount of steps commonplace assay
  • Species reactivity

    Reacts with: Human
bio human baff r

bio human baff r

 

  • Notes

    BAFF-R (moreover usually known as Tumor necrosis draw back receptor superfamily member 13C or B-cell-activating draw back receptor)  is a protein that in of us is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating draw back (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a sort III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

  • Platform

    Pre-coated microplate (12 x Eight appropriately strips)

BIO-X-ACT Short DNA Polymerase

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BIO-X-ACT Short Mix, 2x

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Polyclonal BAFF-R Antibody

APR15125G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications:

BAFF-R Polyclonal Antibody

3578-100
EUR 316

BAFF-R Blocking Peptide

3578RBP-50
EUR 153

BAFF-R Recombinant Protein

90-237 50 ug
EUR 500.75
Description: BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation . Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome , Rheumatoid Arthritis (RA) , Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE) . BAFF is also increased levels in some lymphoid cancers.

Anti-BAFF-R (human) Monoclonal Antibody (11C1)

M02865 100ug
EUR 446
Description: Mouse Monoclonal BAFF-R (human) Antibody (11C1). Validated in Flow Cytometry, IHC and tested in Human.

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GL6265-25MG 25 mg
EUR 447

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GL6265-1MG 1 mg
EUR 126

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GL6265-5MG 5 mg
EUR 195