Precision
| Pattern | n | Degree out | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 147pg/ml | 10.29 | = 7% |
| 2 | 16 | 683pg/ml | 49.85 | = 7.3% |
| 3 | 16 | 2750pg/ml | 206.25 | = 7.5% |
| Pattern | n | Degree out | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 156pg/ml | 13.88 | = 8.9% |
| 2 | 24 | 700pg/ml | 62.3 | = 8.9% |
| 3 | 24 | 2531pg/ml | 227.05 | = 8.9% |
Product overview
The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom-made supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA gear relies upon on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for frequent: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Delicate is added and unbound conjugates are washed away with PBS or TBS buffer.
Key alternatives and particulars
- Sensitivity: 10 pg/ml
- Differ: 78 pg/ml – 5000 pg/ml
- Pattern sort: Cell custom-made supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection approach: Colorimetric
- Assay sort: Sandwich (quantitative)
- Reacts with: Human
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Product prepare
Human BAFF-R ELISA Gadgets -
Detection approach
Colorimetric -
Pattern sort
Cell custom-made supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay sort
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Differ
78 pg/ml – 5000 pg/ml -
Assay time
3h 30m -
Assay measurement
A substantial amount of steps commonplace assay -
Species reactivity
Reacts with: Human
bio human baff r
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Notes
BAFF-R (moreover usually known as Tumor necrosis draw back receptor superfamily member 13C or B-cell-activating draw back receptor) is a protein that in of us is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating draw back (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a sort III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
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Platform
Pre-coated microplate (12 x Eight appropriately strips)
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