Top-of-the-line methods it really works
The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (geared up) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct price to the equivalent earlier curve.
RNA purification and DNase I therapy steps are vital to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, could also be exactly quantified using this gear.
Pay cash for fixed retroviral transductions
A excessive advantage of retroviral gene-delivery strategies is the flexibility to handle the extent of gene expression by adjusting the multiplicity of an an an an infection (MOI) to dictate the number of viral genomes that enter your goal cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell sort and in the long run allow you to administration the expression stage between experiments.
Description
abm’s qPCR Retrovirus Titer Gear is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and could also be achieved beneath one hour. Designed to ship extreme sensitivity and specificity, the gear ensures minimal non-specific background and better full effectivity as in distinction with comparable kits all through the market. ROX reference dye is obtainable separate from the MasterMix, making this gear universally acceptable with most qPCR gadgets.
qPCR and One-Step RT MasterMixes
| PRODUCT COMPONENT |
QUANTITY |
| BlasTaq 2X qPCR Titer MasterMix |
1.25 ml |
| Primer Mix |
100 rxn (200 µl) |
| Commonplace Administration DNA |
50 µl |
| ROX Reference Dye |
15 µl |
| Nuclease-Free H2O |
2 x 1.zero ml |
| SKU |
G949 |
| Unit quantity |
100 rxn |
| Storage State of affairs |
Retailer at -20°C |
|
Virus Titration Kits
Our Titration Kits current a fast and straightforward methodology for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.
Advantages of abm’s titration kits:
- No RNA purification — saves time and eliminates inaccuracy.
- Ready-to-use reagent mix — reduces variability.
- qRT-PCR in a single step — additional delicate and proper than absolutely utterly totally different methods.
- No NTC amplification — Distinctive primer design utterly eliminates non-specific amplification in NTCs.
he Retro-X qRT-PCR Titration Gear provides a fast and straightforward methodology for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.
Quick willpower of retroviral titer
Whereas typical titration methods require as masses as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an an an infection, allowing you to hold out every on the identical day.
 QuickTiter Retrovirus Quantitation Kit |
|
VPK-120 |
Cell Biolabs |
20 assays |
EUR 711 |
|
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus. |
 Retrovirus Mini Purification Kit |
|
K1307-10 |
Biovision |
|
EUR 544 |
Retrovirus Mini Purification Kit |
|
K1307-20 |
Biovision |
|
EUR 914 |
 Retrovirus Maxi Purification Kit |
|
K1308-10 |
Biovision |
|
EUR 1295 |
Retrovirus Maxi Purification Kit |
|
K1308-2 |
Biovision |
|
EUR 533 |
Retrovirus Maxi Purification Kit |
|
K1308-4 |
Biovision |
|
EUR 860 |
 M13 Phage Titration ELISA Kit |
|
55R-PRPHAGE |
Fitzgerald |
96 tests |
EUR 693 |
|
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles |
 Retrovirus Booster 50µl |
|
ABP-RVC-RBST05 |
Allele Biotech |
50µl |
Ask for price |
|
|
 Retrovirus Booster 200µl |
|
ABP-RVC-RBST20 |
Allele Biotech |
200µl |
Ask for price |
|
|
 Retrovirus Booster 400µl |
|
ABP-RVC-RBST40 |
Allele Biotech |
400µl |
Ask for price |
|
|
 Retrovirus Stabilizer 50µl |
|
ABP-RVC-RSTB05 |
Allele Biotech |
50µl |
Ask for price |
|
|
 Retrovirus Stabilizer 200µl |
|
ABP-RVC-RSTB20 |
Allele Biotech |
200µl |
Ask for price |
|
|
 Retrovirus Stabilizer 400µl |
|
ABP-RVC-RSTB40 |
Allele Biotech |
400µl |
Ask for price |
|
|
 Retro-SV40 Retrovirus |
|
G212 |
ABM |
10 ml |
EUR 912 |
 Retro-SV40LT Retrovirus |
|
RVP010 |
ABM |
1.0 ml |
EUR 247 |
 Lentivirus and Retrovirus Purification Kit |
|
K1458-100 |
Biovision |
100 Preps |
EUR 1037 |
Lentivirus and Retrovirus Purification Kit |
|
K1458-20 |
Biovision |
20 Preps |
EUR 606 |
 Entrans qPCR Probe Kit |
|
RK21209 |
Abclonal |
40 RXN |
Ask for price |
|
|
 qPCR Control Template Kit |
|
G929 |
ABM |
100 rxns |
EUR 148 |
 Lentivirus qPCR Quantification Kit |
|
K1471-100 |
Biovision |
100 Rxns |
EUR 298 |
 QPCR Positive Control Kit |
|
M1126-100 |
Biovision |
|
EUR 403 |
 Eva QPCR SuperMix Kit |
|
K5052200 |
Biochain |
200 reactions |
EUR 211 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Eva QPCR SuperMix Kit |
|
K5052400 |
Biochain |
400 reactions |
EUR 320 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Pro QPCR SuperMix Kit |
|
K5053200 |
Biochain |
200 reactions |
EUR 188 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit |
|
K5053400 |
Biochain |
400 reactions |
EUR 299 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
) ViraDuctin Retrovirus Transduction Kit (40 Transductions) |
|
RV-200 |
Cell Biolabs |
40 transductions |
EUR 363 |
|
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells. |
) ViraDuctin Retrovirus Transduction Kit (200 Transductions) |
|
RV-201 |
Cell Biolabs |
200 transductions |
EUR 780 |
|
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells. |
 PCR kit) Endogenous retrovirus (replication competent) PCR kit |
|
PCR-H725-48D |
Bioingentech |
48T |
EUR 453 |
Endogenous retrovirus (replication competent) PCR kit |
|
PCR-H725-96D |
Bioingentech |
96T |
EUR 572 |
