Top-of-the-line methods it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (geared up) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct price to the equivalent earlier curve.

RNA purification and DNase I therapy steps are vital to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, could also be exactly quantified using this gear.

Pay cash for fixed retroviral transductions

A excessive advantage of retroviral gene-delivery strategies is the flexibility to handle the extent of gene expression by adjusting the multiplicity of an an an an infection (MOI) to dictate the number of viral genomes that enter your goal cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell sort and in the long run allow you to administration the expression stage between experiments.

 

Description

abm’s qPCR Retrovirus Titer Gear is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and could also be achieved beneath one hour. Designed to ship extreme sensitivity and specificity, the gear ensures minimal non-specific background and better full effectivity as in distinction with comparable kits all through the market. ROX reference dye is obtainable separate from the MasterMix, making this gear universally acceptable with most qPCR gadgets.

 

qPCR and One-Step RT MasterMixes

qPCR and One-Step RT MasterMixes

PRODUCT COMPONENT QUANTITY
BlasTaq 2X qPCR Titer MasterMix 1.25 ml
Primer Mix 100 rxn (200 µl)
Commonplace Administration DNA 50 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.zero ml

 

SKU G949
Unit quantity 100 rxn
Storage State of affairs Retailer at -20°C

Virus Titration Kits

Our Titration Kits current a fast and straightforward methodology for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than absolutely utterly totally different methods.
  • No NTC amplification — Distinctive primer design utterly eliminates non-specific amplification in NTCs.

he Retro-X qRT-PCR Titration Gear provides a fast and straightforward methodology for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

Quick willpower of retroviral titer

Whereas typical titration methods require as masses as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an an an infection, allowing you to hold out every on the identical day.

 

 

PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500

HS-150-QPCR 100/pk
EUR 318
Description: Sealing Products; Sealing films - Axygen

Retrovirus Packaging Kit

P905 NULL
EUR 0

Retrovirus Mini Purification Kit

K1307-10
EUR 544

Retrovirus Mini Purification Kit

K1307-20
EUR 914

Retrovirus Maxi Purification Kit

K1308-10
EUR 1295

Retrovirus Maxi Purification Kit

K1308-2
EUR 533

Retrovirus Maxi Purification Kit

K1308-4
EUR 860

QuickTiter Retrovirus Quantitation Kit

VPK-120 20 assays
EUR 711
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus.

M13 Phage Titration ELISA Kit

55R-PRPHAGE 96 tests
EUR 693
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles

Retrovirus Booster 50µl

ABP-RVC-RBST05 50µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System

Retrovirus Booster 200µl

ABP-RVC-RBST20 200µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System

Retrovirus Booster 400µl

ABP-RVC-RBST40 400µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System

Retrovirus Stabilizer 50µl

ABP-RVC-RSTB05 50µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System

Retrovirus Stabilizer 200µl

ABP-RVC-RSTB20 200µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System

Retrovirus Stabilizer 400µl

ABP-RVC-RSTB40 400µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System

Retro-SV40LT Retrovirus

RVP010 1.0 ml
EUR 247

Lentivirus and Retrovirus Purification Kit

K1458-100 100 Preps
EUR 1037

Lentivirus and Retrovirus Purification Kit

K1458-20 20 Preps
EUR 606

qPCR Control Template Kit

G929 100 rxns
EUR 148

Eva QPCR SuperMix Kit

K5052200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Eva QPCR SuperMix Kit

K5052400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Pro QPCR SuperMix Kit

K5053400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

QPCR Positive Control Kit

M1126-100
EUR 403

Lentivirus qPCR Quantification Kit

K1471-100 100 Rxns
EUR 298

Endogenous retrovirus (replication competent) PCR kit

PCR-V725-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit

PCR-V725-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

ViraDuctin Retrovirus Transduction Kit (40 Transductions)

RV-200 40 transductions
EUR 363
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

ViraDuctin Retrovirus Transduction Kit (200 Transductions)

RV-201 200 transductions
EUR 780
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.