• Enriching circRNAs in pure samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Discovering out fairly a number of splicing
• Manufacturing of artificial spherical RNAs


RNase R is an E. coli exoribonuclease which displays 3’-to-5’ exonuclease observe, effectively digesting almost all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with non everlasting 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the take a look at of lariat RNA produced by typical splicing, along with circRNAs which come up by means of back-splicing. By eradicating linear
RNAs from cellular or RNA extracts, RNase R drastically facilitates the identification of spherical species by means of RNA-sequencing. This allows researchers to probe the panorama of splicing events with higher depth.


EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Avoid repeated freeze-thaw cycles of all components to retain most effectivity. All components
are frequent for one 12 months from the date of transport when saved and handled exactly.

Biovision rnase-r

Biovision rnase-r


Cat # +Dimension M1228-500
Dimension 500 U
Highlights RNase R is an E. coli exoribonuclease which displays 3’-to-5’ exonuclease observe, effectively digesting almost all linear RNA species.
Storage Conditions -20°C
Present Conditions Gel Pack
USAGE For Evaluation Use Solely! Not For Use in Individuals.


• For digestion of full RNA, longer incubations of 2-Three hours are sometimes required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement further enzyme partway (e.g. 0.5 μl after 1 hour) by means of the tactic. The higher temperature may very well be very useful for degrading terribly structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, because it ought to end in non-enzymatic RNA degradation.

• RNase R displays low observe on tRNA, rRNA and utterly utterly completely different terribly structured RNAs, for which the three’ end is double stranded with a quick 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered observe. If inefficient degradation is observed, it is endorsed to each upscale the digestion, use further RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Perceive that spherical RNAs symbolize a small proportion of full RNA (sometimes 0.1%-0.01%), as a consequence of this actuality RNase R remedy will most positively finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that motive, a starting amount of not lower than 10 µg of full RNA is useful for plenty of downstream capabilities.

• Whereas the enzyme could also be heat inactivated the tactic should not be useful since extreme heat may end up in RNA hurt. Phenol-chloroform precipitation is also utilized as a alternative. For NGS, sturdy half reversible immobilization (SPRI) bead cleanup is useful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum observe. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.

• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Bundle (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)


Fungi/ Yeast Genomic DNA Extraction Mini Kit(50prep)- RNase A

FAFYG001-RNASE 50 preps
EUR 303

Fungi/ Yeast Genomic DNA Extraction Mini Kit(100prep)- RNase A

FAFYG001-1-RNASE 100 preps
EUR 448

RNase A

abx098139-1ml 1 ml
EUR 217

PI / RNAse

abx290029-200tests 200 tests
EUR 272

RNase A

RB0473 500mg
EUR 183.11

RNase A

RN103-001 300mg
EUR 2615

RNase A

EUR 245

Rnase A, Pancreatic

abx082443-25mg 25 mg
EUR 189

DNase (RNase-free)

abx098138-1500U 1500 U
EUR 453

RNase-Free water

abx098145-25ml 25 ml
EUR 175

Rnase (90–105)

5-01885 4 x 1mg Ask for price

RNase A (MBG)

409-050 50 mg
EUR 73

RNase A (MBG)

409-250 250 mg
EUR 193

RNase T2 Antibody

abx237324-100ug 100 ug
EUR 509

RNase 1 Antibody

  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

RNase 13 Antibody

  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul

Rnase A, 1g

EUR 822

Rnase A, 250mg

EUR 281

RiboSafe RNase Inhibitor

BIO-65027 2500 Units Ask for price

RiboSafe RNase Inhibitor

BIO-65028 10,000 Units Ask for price

RNAse H2A Antibody

24819-100ul 100ul
EUR 390

RNase A Solution

G118 1.0 ml; 10 mg/ml
EUR 102

Thermostable RNAse H

EUR 321

RNase III, E.coli

EUR 224

Murine Rnase inhibitor

R301-01 2000 U
EUR 116

Murine Rnase inhibitor

R301-02 10000 U
EUR 166