RNase R is an E. coli

APPLICATIONS:

• Enriching circRNAs in pure samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Discovering out fairly a number of splicing
• Manufacturing of artificial spherical RNAs

DESCRIPTION:

RNase R is an E. coli exoribonuclease which displays 3’-to-5’ exonuclease observe, effectively digesting almost all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with non everlasting 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the take a look at of lariat RNA produced by typical splicing, along with circRNAs which come up by means of back-splicing. By eradicating linear
RNAs from cellular or RNA extracts, RNase R drastically facilitates the identification of spherical species by means of RNA-sequencing. This allows researchers to probe the panorama of splicing events with higher depth.

 

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Avoid repeated freeze-thaw cycles of all components to retain most effectivity. All components
are frequent for one 12 months from the date of transport when saved and handled exactly.

Cat # +Dimension	M1228-500
Dimension	500 U
Highlights	RNase R is an E. coli exoribonuclease which displays 3’-to-5’ exonuclease observe, effectively digesting almost all linear RNA species.
Storage Conditions	-20°C
Present Conditions	Gel Pack
USAGE	For Evaluation Use Solely! Not For Use in Individuals.

SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:

• For digestion of full RNA, longer incubations of 2-Three hours are sometimes required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement further enzyme partway (e.g. 0.5 μl after 1 hour) by means of the tactic. The higher temperature may very well be very useful for degrading terribly structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, because it ought to end in non-enzymatic RNA degradation.

• RNase R displays low observe on tRNA, rRNA and utterly utterly completely different terribly structured RNAs, for which the three’ end is double stranded with a quick 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered observe. If inefficient degradation is observed, it is endorsed to each upscale the digestion, use further RNase R,
or take away rRNA from full RNA extracts earlier to digestion.

• Perceive that spherical RNAs symbolize a small proportion of full RNA (sometimes 0.1%-0.01%), as a consequence of this actuality RNase R remedy will most positively finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that motive, a starting amount of not lower than 10 µg of full RNA is useful for plenty of downstream capabilities.

• Whereas the enzyme could also be heat inactivated the tactic should not be useful since extreme heat may end up in RNA hurt. Phenol-chloroform precipitation is also utilized as a alternative. For NGS, sturdy half reversible immobilization (SPRI) bead cleanup is useful.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum observe. If ETA is
present, compensate by together with MgCl2 to 1.Zero Mm final focus.

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RNase R
M1228-500	Biovision		EUR 365

Fungi/ Yeast Genomic DNA Extraction Mini Kit(50prep)- RNase A
FAFYG001-RNASE	Favorgen	50 preps	EUR 303

Fungi/ Yeast Genomic DNA Extraction Mini Kit(100prep)- RNase A
FAFYG001-1-RNASE	Favorgen	100 preps	EUR 448

RNase A
abx098139-1ml	Abbexa	1 ml	EUR 217
Shipped within 5-10 working days.

PI / RNAse
abx290029-200tests	Abbexa	200 tests	EUR 272
Shipped within 2-3 weeks.

RNase A
M1227-25	Biovision		EUR 245

RNase A
RB0473	Bio Basic	500mg	EUR 183.11
Product category: Proteins/Enzymes/RNase

RNase A
RN103-001	GenDepot	300mg	EUR 2615

RNAse H2A Antibody
24819-100ul	SAB	100ul	EUR 390

Rnase (90–105)
5-01885	CHI Scientific	4 x 1mg	Ask for price

RNase A (MBG)
409-050	GeneOn	50 mg	EUR 73

RNase A (MBG)
409-250	GeneOn	250 mg	EUR 193

DNase (RNase-free)
abx098138-1500U	Abbexa	1500 U	EUR 453
Shipped within 5-10 working days.

RNase-Free water
abx098145-25ml	Abbexa	25 ml	EUR 175
Shipped within 5-10 working days.

RNase 1 Antibody
20-abx141499	Abbexa	EUR 370.00 EUR 606.00 EUR 300.00	100 ul 200 ul 50 ul
Shipped within 5-10 working days.

RNase 13 Antibody
20-abx141582	Abbexa	EUR 370.00 EUR 606.00 EUR 300.00	100 ul 200 ul 50 ul
Shipped within 5-10 working days.

Rnase A, Pancreatic
abx082443-25mg	Abbexa	25 mg	EUR 189
Shipped within 5-10 working days.

RiboSafe RNase Inhibitor
BIO-65027	Bioline	2500 Units	Ask for price

RiboSafe RNase Inhibitor
BIO-65028	Bioline	10,000 Units	Ask for price

RNase T2 Antibody
abx237324-100ug	Abbexa	100 ug	EUR 509
Shipped within 5-12 working days.

RNase A Solution
G118	ABM	1.0 ml; 10 mg/ml	EUR 102

Thermostable RNAse H
M1224-100	Biovision		EUR 321

RNase III, E.coli
M1226-100	Biovision		EUR 224

anti-RNase L
YF-PA24584	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to RNase L

anti-RNase H1
YF-PA26995	Abfrontier	50 ul	EUR 334
Description: Mouse polyclonal to RNase H1

Murine Rnase inhibitor
R301-01	Vazyme	2000 U	EUR 116