Really helpful Assay Procedures
This particle combination (~10×10^6 particles/mL) is beneficial for routine calibration of switch cytometers. Ahead of use, resuspend the particles by vortexing. Dilution of 3-5 drops of particles to 1 ml of sheath fluid will present an ample variety of particles for switch cytometric evaluation.
Description
This product features a single inhabitants of Rainbow Particles which may very well be dyed to a single fluorescent depth. Each Rainbow Particle features a combination of fluorophores which may very well be stably embedded contained within the particle and could be excited at any wavelength from 365-650 nm, permitting most channels in a switch cytometer to be calibrated utilizing the same set of particles. The Rainbow Particles have emission spectra acceptable with many frequent fluorophores used for immunofluorescent staining by switch cytometric evaluation. Rainbow Fluorescent Particles are supplied in a dropper bottle for useful allotting and storage.
These Rainbow Calibration Particles are a combination of three.0 – 3.four μm particles, dyed to 6 absolutely completely completely different fluorescent intensities. As their excitation fluctuate is 365 – 650 nm, they’re usually utilized with most lasers. Nonetheless, they don’t seem to be to be used with the UV laser. The emission spectra of those rainbow particles is appropriate with many frequent fluorophores utilized in immunofluorescent staining with switch cytometric evaluation.
spherotech
Consists of a single peak for optical alignment of any fl ow cytometer in all channels from UV to Far Purple Determines if the fl ow cell is clear and with out fl uidic blockage
• Measures the coeffi cients of variation (CVs), peak channels, and histogram distribution to go looking out out the effectivity of fl ow cytometers.
Product Notices
- Warning: Sodium azide yields terribly poisonous hydrazoic acid beneath acidic circumstances. Dilute azide compounds in working water prior to discarding to keep away from accumulation of most likely explosive deposits in plumbing.
- Cy is a trademark of GE Healthcare.
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