PRODUCT SPECIFICATIONS

DESCRIPTION: Stain kit to quantify total collagen
FORMAT: Glass slides and tissue culture plates
ASSAY TYPE: Dye staining
ASSAY TIME: Varies depending on sample type
STANDARD RANGE: Collagen: 3 μg/section
Non-collagenous proteins: 50 μg/section
NUMBER OF SAMPLES: 30-50 samples/kit
SAMPLE TYPES: Cultured cells and tissue sections
RECOMMENDED SAMPLE DILUTIONS: N/A
CHROMOGEN: N/A (read at 540 and 605 nm)
STORAGE: Room temperature for 12 months

INTRODUCTION

Sirius Red and Fast Green is a dye combination used to distinguish collagen from its surrounding materials. Sirius Red specifically binds the [Gly-X-Y]n helical structure of fibrillar collagens, regardless of collagen type or species, whereas Fast Green binds to non-collagenous proteins. By exploiting the unique features of these two dyes, Chondrex, Inc. provides a simple semi-quantitative assay kit to determine the amounts of collagen and non-collagenous proteins in cultured cell layers and tissue sections (1-2). Because this assay does not require
collagen solubilization, can be used to measure total collagen content in various tissues (3-7).

As Sirius Red and Fast Green have absorptions at 540 nm and 605 nm respectively, the OD values of the extracted dyes can be used to calculate the collagen and non-collagenous protein content of samples. For general histological studies in which tissue sections are 10-20 μm thick, the assay sensitivity for collagen and non-collagenous proteins is greater than 3 μg/section and 50 μg/section, respectively. This kit contains enough reagents to stain 30-50 samples.

 

Chondrex Sirius Red Fast Green Collagen

Chondrex Sirius Red Fast Green Collagen

 

Sirius Red/Fast Green Collagen Staining

The semi-quantitative total tissue collagen detection kit (Sirius Red/Fast Green collagen staining kit: catalog # 9046) provides a simple quantitative micro-assay tool for determining the total amount of collagen and non-collagenous proteins in cultured cell layers and tissue sections by differential staining with two dyes, sirius red and fast green. Sirius Red binds to all types of collagen, whereas fast green stains non-collagenous proteins. This method has been applied to the measurement of collagen contents in various tissues.

Type I Collagen Detection Kits

Type I collagen is a fibrilar collagen consisting of two identical alpha1(I) chains and one alpha2(I) chain. It is the most abundant collagen type and is found in most connective tissues such as skin, bone, tendon, ligament, and heart. Many tissues with the exception of bone, which consists of type I collagen, contain heterotypic fibrils with two or more distinct collagen types in coexistence. In general, type I collagen is dominant and type III collagen is a minor component. Chondrex provides 5 types of type I collagen assay kit for determining bovine, human, mouse, porcine and rat type I collagen.

Type II Collagen Detection Kit

Type II collagen is unique among the collagen family, and its tissue distribution is limited to avascular tissues such as cartilage and the vitreous body of the eyes. Since type II collagen can induce arthritis in experimental animals, autoimmunity to type II collagen is suspected in the pathogenesis of certain autoimmune diseases in humans such as rheumatoid arthritis, eye diseases associated with rheumatoid arthritis, and relapsing polychondritis, which affects specific tissues containing type II collagen. The Type II Collagen Detection Kit (catalog # 6018) is designed to quantify the amount of native type II collagen from various species (such as human, monkey, porcine, bovine, rat, mouse, rabbit, equine, and chick) in cultured tissues or tissue specimens by ELISA.

Semi-Quantitative Total Tissue Collagen Detection Kit (Sirius Red/Fast Green Collagen Staining Kit)

The semi-quantitative total tissue collagen detection kit (Sirius Red/Fast Green collagen staining kit: catalog # 9046) provides a simple quantitative micro-assay tool for determining the total amount of collagen and non-collagenous proteins in cultured cell layers and tissue sections by differential staining with two dyes, sirius red and fast green. Sirius Red binds to all types of collagen, whereas fast green stains non-collagenous proteins. This method has been applied to the measurement of collagen contents in various tissues.

These dyes can be easily extracted from stained tissues, and the amounts (micrograms) of collagen and non-collagenous proteins in each section can be calculated based on OD 540 (sirius red) and OD 605 (fast green). The assay sensitivity is more than sufficient to determine collagen and non-collagenous proteins in 10-20 micrometer tissue sections prepared for general histological studies. The assay sensitivity for collagen is more than 3 micrograms/section, and non-collagenous protein is more than 50 microgram per section.

 

Sirius Red Total Collagen Detection Assay PLATE Kit

9062P 80 samples
EUR 266.05
Description: Sirius Red Total Collagen Detection Assay PLATE Kit

Picro-Sirius Red Solution

SRS250 250 ml
EUR 80

Picro-Sirius Red Solution

SRS500 500 ml
EUR 98

Picro-Sirius Red Solution

SRS999 1000 ml
EUR 143

Concentrating Solution for Sirius Red Total Collagen Detection Kit

90626 50 ml
EUR 67.4
Description: Concentrating Solution for Sirius Red Total Collagen Detection Kit

OPTIONAL GREEN FILTER (572NM)

GDBL-GREEN 1/pk
EUR 1052
Description: Lab Equipment; Axygen Branded EQ

Fast Green FCF

FB0452 5g
EUR 55.22

Fast Green FCF

HY-D0914 500mg
EUR 108

Fast Green FCF

abx082188-5g 5 g
EUR 203

Fast Green Solution

FGN125 125 ml
EUR 66

Fast Green Solution

FGN500 500 ml
EUR 96

Fast Green Solution

FGN999 1000 ml
EUR 124

ViaVac Red/Green

29068 100uL
EUR 261
Description: Minimum order quantity: 1 unit of 100uL

Nuclear Fast Red

AR0008 12mL(for 240 assays)
EUR 65

Nuclear fast red

NB0671 5g
EUR 110.9

Fast-Red Tablet

F3918-002 20TB
EUR 161

Fast-Red Tablet

F3918-005 50TB
EUR 246

25G Fast Green FCF

NAT1348 25G
EUR 160

Fast Red Enhancer for amplifying Fast Red IHC stains, 15ml

NB322 15 ml
EUR 276

Fast Red RC salt

GT3891-25G 25 g
EUR 46

Fast Red KL salt

GT5711-10G 10 g
EUR 122

Fast Red KL salt

GT5711-25G 25 g
EUR 198

Fast Red KL salt

GT5711-5G 5 g
EUR 86

Picro-Sirius Red Stain Kit (For Cardiac Muscle)

SRC-1 1 kit(s)
EUR 103

Fast Green FCF (C.I. 42053)

GT3407-100G 100 g
EUR 229

Fast Green FCF (C.I. 42053)

GT3407-10G 10 g
EUR 62

Fast Green FCF (C.I. 42053)

GT3407-25G 25 g
EUR 94

Fast Green FCF (C.I. 42053)

GT3407-5G 5 g
EUR 54