Introduction Peste des Petits Ruminants Virus (PPRV) is a highly contagious and economically devastating viral disease that primarily affects goats and sheep. Classified as a morbillivirus within the Paramyxoviridae family, PPRV is closely related to rinderpest, measles, and canine distemper viruses. The rapid spread of this virus, its ability to Read more
Polymerase Chain Reaction (PCR) remains the gold standard for molecular detection of Ureaplasma parvum, a species implicated in urogenital tract infections, infertility, and neonatal complications. Standardizing positive controls is a key component in assay validation, especially when deploying protocols across diverse real-time PCR platforms. Importance of Positive Controls in Clinical Read more
Abstract Previously, we developed a single recombinant protein vaccine against plague composed of a fusion between the Fraction 1 (F1) capsular antigen and the V antigen. To determine whether the overall expression, solubility, and recovery of the F1 fusion protein -V could be improved, we modified the original fusion. Standard Read more
Introduction The PhoP/PhoQ two-component signal transduction system is an important regulator of stress responses and virulence in most gram-negative bacteria, but the characterization of PhoP/PhoQ in Shigella has not been thoroughly investigated. In the present study, we found that deletion of phoPQ (ΔphoPQ) from Shigella flexneri Recombinants 2a 301 (Sf301) Read more
Properties Form: lyophilized powder Quality level: 200 Storage temperature: 2-8°C Product Name: Bordetella pertussis Recombinants Genetic information Bordetella pertussis Tohama I … ptxA(2665068) , ptxB(2665069) , ptxC(2665408) General description The product is not activated. If activation is required, see Kaslow, et al., for suggested conditions. Request The toxin is released Read more
Abstract We have implemented a simple, inexpensive and rapid procedure for the validation and performance verification of pipettes mounted on automatic liquid handlers (ALH) as required for ISO 17025 accredited laboratories. A serial dilution of six or seven passages of OrangeG in quadruplicate in a flat-bottom 96-well microtiter plate, manually Read more
Introduction NGeneBio (Seoul, Korea) introduced its next-generation sequencing (NGS)-based oncology/genetic disease kits and NGenePlex nCoV qRT-PCR kit against COVID-19 at AACC 2021. Dedicated to achieving better health through laboratory medicine, the American Association for Clinical Chemistry, AACC, brings together more than 50,000 clinical laboratory professionals, physicians, research scientists, and business Read more
1. Expected use The Novel Coronavirus (2019-nCoV) Nucleic Acid Diagnostic Kit (PCR-Fluorescence Probe) is a real-time RT PCR test intended for the qualitative detection of SARS-CoV-2 nucleic acid in nasopharyngeal swabs, oropharyngeal (throat) swabs, anterior nasal swabs, middle turbinate swabs, nasal washes, and nasal aspirates from individuals suspected of having Read more
Invitrogen dCTP is prepared at a final concentration of 100 mM in highly purified water. 100 mM dCTP is suitable for use in a polymerase chain reaction (PCR), sequencing, stuffing reactions, nick translation, cDNA synthesis, and TdT tracking reactions. Invitrogen’s dNTP manufacturing meets the highest industry standards: 1. Manufactured under Read more
Description It allows the synthesis of cDNA fragments of up to 9 kbp. and include modified bases. It is active within 55°C – 60°C, allows complex RNA templates to be used and provides reaction specificity. It has RNase H activity (suitable for analyzing changes in gene expression). It provides a Read more