Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath pretty a few circumstances could presumably be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could presumably be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in path of pretty a few transcriptional components is broadly demanded.
Product Overview
The ChromaFlash Chromatin Extraction Bundle deal is a whole set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in diverse chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle deal. The isolated chromatin can be utilized in a variety of chromatin-related choices equal to in vitro protein-DNA binding assays and nuclear enzyme assays.
- Terribly fast course of: your full course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
- Helpful and versatile: the bundle is suitable for making ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
- Unsheared chromatin makes it customizable for pretty a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

epigentek chromoplasts chromatin
Principle & Course of
The ChromaFlash Chromatin Extraction Bundle deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the associated temperature.
Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath pretty a few circumstances could presumably be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could presumably be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in path of pretty a few transcriptional components is broadly in demand.
Principle & Course of
The ChromaFlash Plant Chromatin Extraction Bundle deal accommodates your full reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the associated temperature.
Plant Protein Extraction Kit |
BSP004 |
Bio Basic |
20Preps |
EUR 110.9 |
|
Nuclear Protein Extraction Kit |
BSP009 |
Bio Basic |
50Preps |
EUR 89.15 |
|
FFPE Protein Extraction Kit |
BSP058 |
Bio Basic |
50Preps |
EUR 102.2 |
|
EpiQuik Nuclear Extraction Kit |
OP-0002 |
EpiGentek |
100 Assays |
EUR 292.15 |
Description: kits suitable for this type of research |
Quick Gel Extraction Kit |
20-abx098085 |
Abbexa |
|
|
|
Up RNA Extraction Kit |
20-abx098087 |
Abbexa |
|
|
|
Plant RNA extraction Kit |
20-abx098088 |
Abbexa |
|
|
|
Exosomal RNA Extraction Kit |
P200R,-part-of-P200 |
101Bio |
- |
Ask for price |
Exosomal DNA Extraction Kit |
P230-25 |
101Bio |
- |
Ask for price |
Exosomal DNA Extraction Kit |
P230-50 |
101Bio |
- |
Ask for price |
Membrane Protein Extraction Kit |
K2113-50 |
ApexBio |
50 assays |
EUR 544 |
Mammalian Cell Extraction Kit |
K2114-500 |
ApexBio |
500 assays |
EUR 321 |
Membrane Protein Extraction Kit |
K268-50 |
Biovision |
|
EUR 539 |
Mammalian Cell Extraction Kit |
K269-500 |
Biovision |
|
EUR 294 |
Plant Tissue Extraction Kit |
K296-50 |
Biovision |
|
EUR 457 |
Bacterial RNA Extraction Kit |
R403-01 |
Vazyme |
100 rxn |
EUR 155 |
Membrane Protein Extraction Kit |
55R-1357 |
Fitzgerald |
50 assays |
EUR 740 |
Description: Membrane Protein Extraction Kit for use in the research laboratory |
Mammalian Cell Extraction Kit |
55R-1358 |
Fitzgerald |
500 assays |
EUR 381 |
Description: Mammalian Cell Extraction Kit for use in the research laboratory |
Total RNA Extraction Kit |
K2014005 |
Biochain |
1 kit |
EUR 344 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Total Protein Extraction Kit |
K3011010 |
Biochain |
1 kit |
EUR 370 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Membrane Protein Extraction Kit |
K3014005 |
Biochain |
1 kit |
EUR 382 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Genomic DNA Extraction Kit |
K5016005 |
Biochain |
1 kit |
EUR 329 |
Frit Kit |
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
Triton Extraction Buffer(TEB), for Histon Extraction |
T8300-100 |
GenDepot |
100ml |
EUR 126 |