Background Information

Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath pretty a few circumstances could presumably be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could presumably be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in path of pretty a few transcriptional components is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle deal is a whole set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in diverse chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle deal. The isolated chromatin can be utilized in a variety of chromatin-related choices equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

• Terribly fast course of: your full course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
• Helpful and versatile: the bundle is suitable for making ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
• Unsheared chromatin makes it customizable for pretty a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

epigentek chromoplasts chromatin

Principle & Course of

The ChromaFlash Chromatin Extraction Bundle deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the associated temperature.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath pretty a few circumstances could presumably be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could presumably be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in path of pretty a few transcriptional components is broadly in demand.

Plant Protein Extraction Kit
BSP004	Bio Basic	20Preps	EUR 110.9

Nuclear Protein Extraction Kit
BSP009	Bio Basic	50Preps	EUR 89.15

FFPE Protein Extraction Kit
BSP058	Bio Basic	50Preps	EUR 102.2

EpiQuik Nuclear Extraction Kit
OP-0002	EpiGentek	100 Assays	EUR 292.15
Description: kits suitable for this type of research

Quick Gel Extraction Kit
20-abx098085	Abbexa	EUR 286.00 EUR 203.00	200 rxns 50 rxns

Up RNA Extraction Kit
20-abx098087	Abbexa	EUR 258.00 EUR 105.00	100 ml 3 ml

Plant RNA extraction Kit
20-abx098088	Abbexa	EUR 258.00 EUR 105.00	100 ml 3 ml

Exosomal RNA Extraction Kit
P200R,-part-of-P200	101Bio	-	Ask for price

Exosomal DNA Extraction Kit
P230-25	101Bio	-	Ask for price

Exosomal DNA Extraction Kit
P230-50	101Bio	-	Ask for price

Membrane Protein Extraction Kit
K2113-50	ApexBio	50 assays	EUR 544

Mammalian Cell Extraction Kit
K2114-500	ApexBio	500 assays	EUR 321

Membrane Protein Extraction Kit
K268-50	Biovision		EUR 539

Mammalian Cell Extraction Kit
K269-500	Biovision		EUR 294

Plant Tissue Extraction Kit
K296-50	Biovision		EUR 457

Bacterial RNA Extraction Kit
R403-01	Vazyme	100 rxn	EUR 155

Membrane Protein Extraction Kit
55R-1357	Fitzgerald	50 assays	EUR 740
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit
55R-1358	Fitzgerald	500 assays	EUR 381
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Total RNA Extraction Kit
K2014005	Biochain	1 kit	EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein Extraction Kit
K3011010	Biochain	1 kit	EUR 370
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein Extraction Kit
K3014005	Biochain	1 kit	EUR 382
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Genomic DNA Extraction Kit
K5016005	Biochain	1 kit	EUR 329

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Triton Extraction Buffer(TEB), for Histon Extraction
T8300-100	GenDepot	100ml	EUR 126

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