Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is often used for blotting, immunoassays and immunohistochemistry strategies. It’s a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, leading to a coloured or fluorescent product or launch of sunshine as a byproduct of the response. This product is on the market in a stabilized liquid sort.For substrate use of HRP-labelled protein detection we provide our Intensive ChemiLuminescence (ICLTM) gear (SKU: 20810000). ICL is a two-component substrate that accommodates a gradual luminol reply with an enhancer and a gradual peroxide buffer reply.

It has a strong gentle emission and a sensitivity at picogram diploma that’s terribly acceptable for the speedy detection of HRP-conjugated proteins. Benefits of chemiluminescent substrates over utterly totally different detection strategies includes varied exposures, blots can also be reprobed, detects and quantitates various protein concentrations, and yields largest sensitivity of any in the marketplace technique.We furthermore present 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection.

Summary

Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker  it strongly recognises primarily probably the most cancers particular T antigen (Galbeta1–>3GalNAc-), nonetheless not its sialylated model. Nonetheless, an extra specificity inside the route of Galbeta1–>4GlcNAc (LacNAc), which isn’t tumour particular, had been attributed to PNA.

For proper interpretation of lectin histochemical outcomes we examined PNA sugar specificity utilizing naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, pretty than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and utterly totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, together with selective eradicating of O-linked oligosaccsharides and treatment with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) together with uncovered T antigen (Galbeta1–>Three GalNAc-) was improbable as sugar moiety in glycoproteins for his or her recognition by PNA.

 

arachis eylabs

arachis eylabs

Software program program

Widespread Western Blot Protocol:

  • Glycoprotein pattern dimension: 500ng
  • Lectin Focus: 0.1ug/ml
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS web internet web page gel
  3. Change gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for two hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect utilizing chemiluminescent substrate (CPS1-120)

Biochem/Physiol Actions

PNA wouldn’t agglutinate frequent human erythrocytes, nonetheless strongly agglutinates neuraminidase handled erythrocytes. PNA has potent anti-T prepare very just like the anti-T antibody in human sera. The lectin may be utilized to tell apart between human lymphocyte subsets.

Unit Definition

One unit will sort 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Bodily sort

Accommodates sodium citrate

Arachis hypogaea lectin (PNA

05-0116-50 50 mg
EUR 314

Arachis hypogaea Conglutin-7

1-CSB-EP757666ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 22 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Conglutin-7 expressed in E.coli

Arachis hypogaea Arachin Ahy-3

1-CSB-EP737391ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 35.9 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli

Arachis hypogaea Arachin Ahy-3

1-CSB-YP737391ANE
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 21.9 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-EP331779ANE(A4)
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 71.7 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-EP331779ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 39 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-YP331779ANE(A4)
  • EUR 586.00
  • EUR 299.00
  • EUR 2172.00
  • EUR 900.00
  • EUR 1442.00
  • EUR 382.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 70.2 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P41B

1-CSB-CF337356ANE
  • EUR 965.00
  • EUR 665.00
  • EUR 715.00
  • 1MG
  • 200ug
  • 500ug
  • MW: 72.8 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system

Biotin [HRP]

DAG3197-HRP 1mg
EUR 429

Vitamine B9 [HRP]

DAGDT1440-HRP 1mg
EUR 1105

Vitamine B12 [HRP]

DAGT5413-HRP 1mg
EUR 975

GAPDH Antibody - HRP

T0004-HRP 1ml
EUR 920
Description: GAPDH Mouse Monoclonal antibody detects endogenous levels of total GAPDH protein.

CF488A Arachis hypogaes Lectin PNA (Peanut)

29060 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF568 Arachis hypogaes Lectin PNA (Peanut)

29061 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF594 Arachis hypogaes Lectin PNA (Peanut)

29062 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF640R Arachis hypogaes Lectin PNA (Peanut)

29063 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

HRP-IgG Conjugation Kit

55R-HRP 1 mg
EUR 705
Description: HRP conjugation kit for all types of IgG

beta-Actin Antibody - HRP

T0022-HRP 1ml
EUR 920
Description: beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.