mCherry is the second generation monomeric red fluorescent protein that was derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), such as GFP or DsRed. mCherry is widely used as a fluorescent tracer in transfection and transgenic experiments because of its better performance in brightness, photostability and monomeric structure. Since mCherry requires no additional substrates or cofactors, mCherry can be easily detected under a fluorescence microscope.
However, most imaging studies of mCherry are only qualitative. BioVision’s mCherry Quantification Kit provides an easy 96 micro-plate assay to analyze mCherry expression level in cells or tissues quantitatively. Cells or tissues can be homogenized directly in the mCherry Assay Buffer. The amount of mCherry is determined by comparing its fluorescence (Ex/Em= 587/610 nm) with a mCherry standard that is provided in this kit. Each kit provides sufficient reagents to perform up to 100 assays.
mCherry Quantification Kit
IHC using an anti-mCherry antibody (fluorescent and stained image). Intestinal tissue from a transgenic mouse expressing mCherry in all tissues. (A) Fluorescent image detecting mCherry expression and (B) HRP-stained image. Following isolation and fixation in 4% paraformaldehyde, mCherry Monoclonal Antibody (16D7) (Cat. No. M11217) was used at a 1:15,000 dilution. The ImmPRESS™ Anti-Rat Ig (peroxidase) Polymer Detection Kit (Vector Laboratories) was used according to manufacturer’s instructions; sections were incubated in peroxidase substrate solution until the desired stain intensity developed.
Flow cytometry analysis of cells expressing mCherry. U20S cells expressing mCherry were analyzed using 405 nm excitation and 450/40 nm band pass emission on an Attune Acoustic Focusing Cytometer. The histogram shows cells stained with mCherry rat monoclonal antibody conjugated with Pacific Blue (black line) and unstained cells (gray line).
| Cat # +Size |
K971-100 |
| Size |
100 assays |
| Detection Method |
Fluorescence (Ex/Em 587/610 nm) |
| Species Reactivity |
Mammalian |
| Applications |
It detects a wide range of mCherry (0.01-1 ng/µl) |
| Features & Benefits |
• Simple procedure; takes ~ 20 minutes
• Fast and convenient |
| Kit Components |
• mCherry Assay Buffer
• mCherry Standard |
| Storage Conditions |
-20°C |
| Shipping Conditions |
Gel Pack |
| USAGE |
For Research Use Only! Not For Use in Humans. |
mCherry Antibody (PA5-34974) in WB
Western Blot analysis of mCherry was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a mCherry Polyclonal Antibody (Product # PA5-34974) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
 mCherry antibody |
|
70R-12458 |
Fitzgerald |
100 ug |
EUR 527 |
|
Description: Rabbit polyclonal mCherry antibody |
 mCherry-Tag Antibody |
|
1-CSB-PA000343 |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against mCherry-Tag. Recognizes mCherry-Tag from . This antibody is Unconjugated. Tested in the following application: WB;WB:1:5000 |
 Anti-mCherry antibody |
|
STJ140000 |
St John's Laboratory |
300 µg |
EUR 270 |
|
Description: Goat polyclonal antibody to mCherry (Cherry fluorescent protein). |
Anti-mCherry antibody |
|
STJ140001 |
St John's Laboratory |
300 µg |
EUR 270 |
|
Description: Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. |
 mCherry Monoclonal Antibody |
|
ABM40125-003ml |
Abbkine |
0.03ml |
EUR 158 |
|
|
|
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein |
mCherry Monoclonal Antibody |
|
ABM40125-01ml |
Abbkine |
0.1ml |
EUR 289 |
|
|
|
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein |
mCherry Monoclonal Antibody |
|
ABM40125-02ml |
Abbkine |
0.2ml |
EUR 414 |
|
|
|
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein |
 mCherry-tag Antibody |
|
20-abx134448 |
Abbexa |
-
EUR 356.00
-
EUR 537.00
-
EUR 217.00
|
|
|
|
mCherry-tag Antibody |
|
20-abx134449 |
Abbexa |
-
EUR 356.00
-
EUR 537.00
-
EUR 217.00
|
|
|
|
mCherry-tag Antibody |
|
20-abx134450 |
Abbexa |
-
EUR 356.00
-
EUR 537.00
-
EUR 217.00
|
|
|
|
 mCherry tag Antibody |
|
abx019132-100ug |
Abbexa |
100 ug |
EUR 356 |
|
|
mCherry-Tag Antibody |
|
20-abx242885 |
Abbexa |
|
|
|
|
mCherry-Tag Antibody |
|
20-abx330249 |
Abbexa |
|
|
|
|
mCherry-Tag Antibody |
|
20-abx330282 |
Abbexa |
|
|
|
|
Anti-mCherry antibody |
|
STJ97027 |
St John's Laboratory |
200 µl |
EUR 197 |
|
Description: Mouse monoclonal to mCherry. |
mCherry-Tag Antibody |
|
T0090 |
Affbiotech |
1ml |
EUR 920 |
|
Description: The mCherry tag antibody can recognize mCherry and mCherry-tag fusion proteins. |
 mCherry - Mouse |
|
MO22140 |
Neuromics |
100 ul |
EUR 435 |
 mCherry-Rabbit |
|
RA22117 |
Neuromics |
100 ul |
EUR 435 |
 mCherry-Chicken |
|
CH22115 |
Neuromics |
100 ul |
EUR 435 |
