Primarily primarily most likely essentially the most normally used histological stain, hematoxylin is a pure compound extracted from a species of tree current in Mexico and the West Indies. The extracted compound is oxidized to offer hematein, the energetic staining ingredient of the hematoxylin stain.
The manufacturing of histological staining alternatives is an work. Just some laboratories are capable of making a stain of reliable vibrance. At Nationwide Diagnostics we’ve now now now gone one step extra.

Histo-Clear II

  • Low Hazard
  • Biodegradable
  • Lowered Citrus Odor

Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that ends in the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and fully biodegradable, thus decreasing disposal costs. Histo-Clear II has a vastly diminished citrus odor as in distinction with totally completely totally different citrus-based histological clearing brokers.

Uncover: Histo-Clear II is simply not relevant with many synthetic mounting media. Histo-Clear II is a long-standing favorite dry mounting procedures and aqueous mounting. Please affirm compatibility alongside collectively collectively together with your mounting media earlier than using Histo-Clear II with in wet-mounting with synthetic media.

 

Hazardous Provides Worth (HazMat) applies if shipped by Air.

PROCEDURE

Uncover: The next protocol has given good to good outcomes with every kind of tissue sections. It
would possibly ought to be optimized for a given tissue, half
thickness, and so forth.

National Diagnostics HISTO-CLEAR

Nationwide Diagnostics HISTO-CLEAR

1. Dewax and rehydrate slides

a) Immerse slides inside the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes

2. Stain
a) Immerse slides in Hematoxylin reply for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for 2 minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.

3. Dehydrate, clear and mount
a) Immerse slides inside the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.

 

Histo-Clear II

NAT1334 1 US gallon
EUR 126

Histo-Clear II

NAT1336 5 US gallons
EUR 381

Calci-Clear

NAT1312 EACH
EUR 78

Calci-Clear

NAT1314 EACH
EUR 128

Calci-Clear

NAT1316 EACH
EUR 386

Calci-Clear Rapid

NAT1318 EACH
EUR 75

Calci-Clear Rapid

NAT1320 EACH
EUR 128

Calci-Clear Rapid

NAT1322 EACH
EUR 386

10X PBS MaxTag Histo (OKRA00043)

OKRA00043 100 ml
EUR 140
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Hematoxylin Counterstain MaxTag Histo (OKRA00050)

OKRA00050 15 ml
EUR 140
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Streptavidin-Peroxidase Conjugate MaxTag Histo (OKRA00036)

OKRA00036 1 each
EUR 283
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

PolyMount Mounting Media MaxTag Histo (OKRA00045)

OKRA00045 15 ml
EUR 126
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Diogenes Kit

NAT1000 1KIT
EUR 225

Hydrogen Peroxide Assay Kit

NAT1002 1KIT
EUR 257

ProtoBlock System

NAT1004 1KIT
EUR 102

450ML PBS 10X

NAT1006 450ML
EUR 80

4L PBS 10X

NAT1008 4L
EUR 199

ProtoGlow ECL 200ml Kit

NAT1010 EACH
EUR 295

ProtoGlow ECL 500ml Kit

NAT1012 EACH
EUR 523

100G Acrylamide

NAT1014 100G
EUR 100

500G Acrylamide

NAT1016 500G
EUR 218

1KG Acrylamide

NAT1018 1KG
EUR 344

25G AquaPor LE

NAT1020 25G
EUR 110

100G AquaPor LE GTAC

NAT1022 100G
EUR 209

500G AquaPor LE GTAC

NAT1024 500G
EUR 732

25G AquaPor ES

NAT1026 25G
EUR 127