AffiAAV® is renowned for its pioneering efforts in the field of gene therapy and genetic research. We specialize in producing a comprehensive range of recombinant Adeno-Associated Virus (rAAV) vectors, which are instrumental in delivering genetic material into cells for various research purposes. Explore some of our standout products and their applications below.
Our Premier rAAV Vectors
rAAV-hSyn-Jaws-eGFP-er2-wpre-pa
- Catalog Number: AFG-BRV-1354
- Description: This vector features the human Synapsin (hSyn) promoter, which drives the expression of Jaws, a light-sensitive protein, fused with enhanced Green Fluorescent Protein (eGFP). The inclusion of Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) and a polyadenylation (PA) sequence ensures enhanced stability and efficiency of gene expression.
- Applications: Ideal for optogenetics, this vector is used in neuronal studies to control and modulate neural activity using light. The eGFP tag allows for visualization and tracking of expression in neurons.
rAAV-mth-archt-mCherry-wpres
- Catalog Number: AFG-BRV-1343
- Description: Incorporates the methionine tRNA synthetase (mth) promoter to drive the expression of ArchT, a proton pump used for optogenetic silencing of neurons, fused with the fluorescent protein mCherry. The vector also includes WPRE sequences to enhance gene expression levels.
- Applications: This vector is essential for optogenetic studies aimed at silencing neurons, allowing researchers to investigate and understand neural circuits and their functions.
rAAV-CMV-wpre-hgh-polya
- Catalog Number: AFG-BRV-0060
- Description: Utilizes the Cytomegalovirus (CMV) promoter known for its robust and high-level gene expression capabilities. This vector includes WPRE for enhanced expression and a human growth hormone (hGH) polyadenylation (polyA) tail for mRNA stability.
- Applications: Suitable for a wide range of gene expression studies across various cell types, making it a versatile tool for researchers.
rAAV-CAG-h2b-tdTomato-wpre-hgh-polya
- Catalog Number: AFG-BRV-0042
- Description: This vector features the CAG promoter, which drives high-level expression of the histone H2B protein fused with the fluorescent protein tdTomato. The WPRE and hGH polyA sequences enhance expression and stability.
- Applications: Useful for cell labeling and tracking, particularly in developmental studies where precise and stable expression is required.
rAAV-hSyn-mCherry-wpre-hgh-polya
- Catalog Number: AFG-BRV-0019
- Description: Driven by the hSyn promoter for neuron-specific expression, this vector encodes mCherry, a red fluorescent protein, with WPRE and hGH polyA for enhanced expression and stability.
- Applications: Primarily used in neuroscience for tracking and imaging neuronal cells, facilitating studies on neuronal structure and function.
rAAV-ef1a-dio-bfp-flag-wpre-bgh-polya
- Catalog Number: AFG-BRV-0210
- Description: Features the EF1a promoter driving Cre-dependent expression of BFP-Flag, along with WPRE and BGH polyA for improved gene expression and stability.
- Applications: Ideal for conditional expression studies utilizing the Cre-loxP system, allowing for precise control of gene expression in target cells.
rAAV-vGAT1-dio-eGFP-wpre-hgh-polya
- Catalog Number: AFG-BRV-0256
- Description: Uses the vesicular GABA transporter (vGAT1) promoter to drive Cre-dependent expression of eGFP, with WPRE and hGH polyA for enhanced stability and expression.
- Applications: Ideal for studies focusing on inhibitory neurons, enabling researchers to visualize and analyze the function and distribution of GABAergic neurons.
rAAV-scaav-hsyn-fdio-egfp-wpres
- Catalog Number: AFG-BRV-0273
- Description: This self-complementary AAV vector features the hSyn promoter for neuron-specific, Cre-dependent expression of eGFP, enhanced by WPRE sequences.
- Applications: Useful in rapid expression studies and for mapping neuronal connectivity, providing high-efficiency transduction in target neurons.
Cutting-Edge Science and Biotechnology, Laboratory Research, Cell Culture, and Medical Discoveries. Cells Biological Culture Concept. Cell Culturing At The Safety Cabinet, Doing a Research In The laboratory.
rAAV-CMV-eYFP-wpre-hgh-polya
- Catalog Number: AFG-BRV-0069
- Description: Incorporates the CMV promoter for robust expression of eYFP, with WPRE and hGH polyA for enhanced expression and mRNA stability.
- Applications: Versatile for high-efficiency expression in various cell types, facilitating a wide range of gene expression studies.
rAAV-hSyn-eYFP-wpre-bgh-polya
- Catalog Number: AFG-BRV-0034
- Description: Driven by the hSyn promoter for neuron-specific expression of eYFP, including WPRE and BGH polyA for enhanced gene expression and stability.
- Applications: Essential for visualizing and studying neuronal structures, aiding in the analysis of neural circuits and pathways.
rAAV-CAG-flex-archt-eGFP-wpre-sv40-polya
- Catalog Number: AFG-BRV-1348
- Description: Utilizes the CAG promoter and FLEX technology for Cre-dependent expression of ArchT and eGFP, with WPRE and SV40 polyA for stability.
- Applications: Suitable for optogenetic silencing and visualization in Cre-expressing cells, facilitating studies on neuronal inhibition and circuit analysis.
rAAV-PV-eGFP-bgh-polya
- Catalog Number: AFG-BRV-0075
- Description: Contains the parvalbumin (PV) promoter for PV neuron-specific expression of eGFP, with BGH polyA for mRNA stability.
- Applications: Useful in studies focusing on parvalbumin-expressing interneurons, enabling detailed visualization and analysis of these cells.
rAAV-CAG-dio-tdTomato-wpre-hgh-polya
- Catalog Number: AFG-BRV-0243
- Description: Features the CAG promoter for Cre-dependent tdTomato expression, with WPRE and hGH polyA for enhanced gene expression and stability.
- Applications: Ideal for lineage tracing and neuronal mapping, providing bright and stable fluorescent labeling.
rAAV-CamKIIa-quasar2-mOrange-wpre-hgh-polya
- Catalog Number: AFG-BRV-1360
- Description: Features the CamKIIa promoter for specific expression in excitatory neurons, with Quasar2-mOrange for fluorescent labeling, and includes WPRE and hGH polyA for stability.
- Applications: Suitable for activity-dependent labeling and optogenetic studies, allowing researchers to visualize and manipulate excitatory neurons.
These vectors represent just a subset of our extensive catalog, each designed to cater to specific research needs. Whether for gene therapy, neuronal mapping, or optogenetic control, AffiAAV® provides the tools necessary to advance scientific discovery.
For more detailed information and to explore additional products, visit the website and browse through our comprehensive range of rAAV vectors and related resources.