Choices

Additional Delicate than MTT, MTS, or WST-1

No Toxicity to Cell

Easier Steps, No pure solvents required

Frequent One Bottle Reply, ready-to-use

Background

Cell proliferation will possibly be detected by fairly just a few methods. Cell proliferation induces an increase all by means of the follow of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt to formazan.

The WST Cell Proliferation Colorimetric Assay Bundle deal plus offers among the many best and most delicate method for quantification of cell proliferation and viability. Cell proliferation causes the rise all by means of the amount of formazan dye common that is straight proportional to the number of residing cells and might possibly be quantified by measuring the absorbance of the dye decision at 440 nm using microtiter plate reader. The gear may be utilized for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer remedy and loads of fully completely completely different toxic brokers.

The assay may be used for the detection of cell proliferation in response to cytokines, mitogens, progress parts and dietary dietary nutritional vitamins, and so forth. The assay would possibly very properly be very simple and simple, merely add-and-read, requiring no solubilization, no washing and no harvesting steps, and is widespread, sooner and additional delicate than MTS, MTT or XTT-based assays. Your full assay will possibly be carried out in a microtiter plate and correlates accurately with the [3H]-thymidine incorporation assay.

Description

Our product Cell Counting Bundle deal-8 (CCK-8) current a further helpful and delicate technique for the evaluation of cell amount dedication and cell proliferationtotoxicity assay than earlier strategies. The gear makes use of a really water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon low value all by means of the presence of an electron mediator. The amount of the formazan generated by dehydrogenases is straight in proportion to the numbers of residing cells. The detection sensitivity by CCK-Eight is bigger than fully completely completely different tetrazolium salts resembling MTT, XTT, MTS or WST-1.

Cell Counting Kit-8 Apexbio

Cell Counting Equipment-Eight Apexbio

Choices & Properties

Choices Fast and helpful Straightforward one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The method could be very straightforward that requires no washing/harvesting/or solubilization steps and is faster and additional delicate than MTT/XTT/or MTS-based assays. Your full assay will possibly be carried out straight in a 96-well plate.
Present Gel pack
Storage Circumstances Retailer at -20°C.
Apexbio Cell Counting Kit-8

Apexbio Cell Counting Equipment-8

 

 

[Linking template=”default” type=”products” search=”apexbio cell counting kit” header=”2″ limit=”22″ start=”3″ showCatalogNumber=”true” showSize=”true” showSupplier=”true” showPrice=”true” showDescription=”true” showAdditionalInformation=”true” showImage=”true” showSchemaMarkup=”true” imageWidth=”” imageHeight=””]