Background Information

Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath pretty a few circumstances could presumably be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could presumably be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in path of pretty a few transcriptional components is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle deal is a whole set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in diverse chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle deal. The isolated chromatin can be utilized in a variety of chromatin-related choices equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your full course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Helpful and versatile: the bundle is suitable for making ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for pretty a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin

epigentek chromoplasts chromatin

Principle & Course of

The ChromaFlash Chromatin Extraction Bundle deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the associated temperature.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath pretty a few circumstances could presumably be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could presumably be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in path of pretty a few transcriptional components is broadly in demand.

Principle & Course of
The ChromaFlash Plant Chromatin Extraction Bundle deal accommodates your full reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the associated temperature.
ExoDNA? Extraction Kit
K1242-50
EUR 881
miRNA Extraction Kit
K1456-50 50 Preps
EUR 551
Quick Gel Extraction Kit
20-abx098085
  • EUR 286.00
  • EUR 203.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.
Up RNA Extraction Kit
20-abx098087
  • EUR 258.00
  • EUR 105.00
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.
Plant RNA extraction Kit
20-abx098088
  • EUR 258.00
  • EUR 105.00
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.
Membrane Protein Extraction Kit
55R-1357 50 assays
EUR 740
Description: Membrane Protein Extraction Kit for use in the research laboratory
Mammalian Cell Extraction Kit
55R-1358 500 assays
EUR 381
Description: Mammalian Cell Extraction Kit for use in the research laboratory
Total RNA Extraction Kit
K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein Extraction Kit
K2113-50 50 assays
EUR 544
Mammalian Cell Extraction Kit
K2114-500 500 assays
EUR 321
Membrane Protein Extraction Kit
K268-50
EUR 539
Mammalian Cell Extraction Kit
K269-500
EUR 294
Total Protein Extraction Kit
K3011010 1 kit
EUR 370
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Membrane Protein Extraction Kit
K3014005 1 kit
EUR 382
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Plant Tissue Extraction Kit
K296-50
EUR 457
EpiQuik Nuclear Extraction Kit
OP-0002 100 Assays
EUR 292.15
Description: kits suitable for this type of research
Genomic DNA Extraction Kit
K5016005 1 kit
EUR 329
Exosomal RNA Extraction Kit
P200R,-part-of-P200 NULL
EUR 0
Exosomal DNA Extraction Kit
P230-25 NULL
EUR 0
Exosomal DNA Extraction Kit
P230-50 NULL
EUR 0
Bacterial RNA Extraction Kit
R403-01 100 rxn
EUR 155
Total Protein Extraction Kit
BSP003 50Preps
EUR 109.16
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal
Plant Protein Extraction Kit
BSP004 20Preps
EUR 110.9
  • Product category: Molecular Biology Kits/Protein - Extraction/Plant
Nuclear Protein Extraction Kit
BSP009 50Preps
EUR 89.15
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal