Background Information

Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath pretty a few circumstances could presumably be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could presumably be detected by ChIP-chip. Notably, the ChIP methodology with explicit antibodies straight in path of pretty a few transcriptional components is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Bundle deal is a whole set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle may be utilized in diverse chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Bundle deal. The isolated chromatin can be utilized in a variety of chromatin-related choices equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your full course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Helpful and versatile: the bundle is suitable for making ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for pretty a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin

epigentek chromoplasts chromatin

Principle & Course of

The ChromaFlash Chromatin Extraction Bundle deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the associated temperature.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. As an illustration, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath pretty a few circumstances could presumably be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale could presumably be detected by ChIP-chip. Notably, a ChIP methodology with explicit antibodies straight in path of pretty a few transcriptional components is broadly in demand.

Principle & Course of
The ChromaFlash Plant Chromatin Extraction Bundle deal accommodates your full reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the associated temperature.
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