Lipid peroxidation assay protocol summary:

– add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– swap to wells of microplate
– analyze with microplate reader
For greater sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis.

Product overview

Lipid Peroxidation (MDA) Assay Bundle (Colorimetric/Fluorometric) (ab118970) offers a helpful software program program program for delicate detection of malondialdehyde (MDA).

Contained within the lipid peroxidation assay protocol, the MDA contained within the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct could also be merely quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/effectively colorimetrically and 0.1 nmol/effectively fluorometrically.

The MDA assay will even be refered to as a TBARS assay.

lipid-peroxidation-assay-kit

lipid-peroxidation-assay-kit

Lipid Peroxidation (MDA + HNE) Assay Bundle

Lipid peroxidation is a extensively acknowledged occasion of oxidative hurt in cell membranes, lipoproteins, and fully fully completely different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl ldl ldl cholesterol can occur in quite a few reactions. They’re normally triggered by i) free radical species equal to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low worth of hydrogen peroxide or ii) non-radical species equal to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are essential toxic byproducts of lipid peroxidation. So, the measurement of the elements of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a necessary product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, elevated ranges of HNE had been current in plasma and quite a few organs beneath oxidative stress circumstances. The very fact is, MDA is in a great deal of circumstances virtually actually principally most likely essentially the most ample explicit explicit individual aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and an excessive amount of fully fully completely different biomolecules.

Bioquochem’s LPO assay devices measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). As a consequence of this, this response offers a diindolylalkane (chromophore) with most absorbance contained within the space of 580-620 nm.

 

Lipid Peroxidation biovision

Lipid Peroxidation biovision

In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a extreme molar extinction coefficient at its maximal absorption wavelength of 586 nm.

Chinese language language language language protocol within the market. See protocols half beneath.

For an alternate MDA assay, with out the heating steps required contained within the TBARS assay, try MDA assay ab233471.

lipid-peroxidation-assay-kit

lipid-peroxidation-assay-kit

  • Notes

    Lipid peroxidation refers as soon as extra to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (sometimes in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is necessary to guage oxidative stress. Lipid peroxidation varieties reactive aldehydes equal to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are normally used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.

    Related merchandise

    Evaluation the oxidative stress marker and assay information, or the overall metabolism assay information to look at further assays for metabolites, metabolic enzymes, mitochondrial carry out, and oxidative stress, and along with how one can assay metabolic carry out in dwell cells using your plate reader.

    Moreover see the favored 4-HNE Assay Bundle ab238538 in its place marker of lipid peroxidation and oxidative stress.


    How fully fully completely different researchers have used Lipid Peroxidation Assay Bundle ab118970

    The MDA/TBARs assay devices has been utilized in publications in numerous sample varieties, along with:
    – Human: serum1, hippocampal principal cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4
    – Mouse: neuronal cell lysates5, coronary coronary coronary coronary heart tissue extract6, plasma7, cell extracts8
    – Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11
    – Pig: serum12

    References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019

  • Platform

    Microplate reader
Lipid Peroxidation Colorimetric Assay Kit
K454-100
EUR 544
U-74389G (Lipid peroxidation blocker)
SIH-205-100MG 100 mg
EUR 174
  • U-74389G is an antioxidant which prevents iron-dependent lipid peroxidation. It prevents organ damage in intestinal cold storage, preservation and reperfusion injury (1-4).
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.
U-74389G (Lipid peroxidation blocker)
SIH-205-500MG 500 mg
EUR 582
  • U-74389G is an antioxidant which prevents iron-dependent lipid peroxidation. It prevents organ damage in intestinal cold storage, preservation and reperfusion injury (1-4).
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.
Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K2167-100 100 assays
EUR 586
Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K739-100
EUR 582
Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
22906 200 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
15991 200 tests
EUR 393
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
K747-100
EUR 327
OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric)
STA-805 192 assays
EUR 577
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample.
Lipid A Lipopolysaccharide (LPS Lipid A / Endotoxin) Antibody
abx415762-1ml 1 ml
EUR 606
  • Shipped within 1 week.
Lipid A Antibody
abx023083-1ml 1 ml
EUR 739
  • Shipped within 5-10 working days.
Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free)
MET-5009-C 50 preps
EUR 659
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis.
Ceraplex Lipid Concentrate(1000X)
C3316-010 100ml
EUR 120
Ceraplex Lipid Concentrate(1000X)
C3316-050 500ml
EUR 246
Lipid Droplet Isolation Kit
MET-5011 50 preps
EUR 415
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.
Lipid Quantitation Kit (Colorimetric)
STA-613 100 assays
EUR 531
Description: Cell Biolabs? Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method, resulting in a simple colorimetric readout amenable to multi-well plate detection.
Lipid Quantification Kit (Fluorometric)
STA-617 100 assays
EUR 531
Description: The Lipid Quantification Kit (Fluorometric) uses a fluorometric detection system to quantify neutral lipids in a convenient 96-well microplate reader. The kit includes sufficient reagents for 100 assays.
Monophosphoryl Lipid A (Synthetic)
VAdv-Ly0012-1mg 1 mg
EUR 861
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.
Monophosphoryl Lipid A (Synthetic)
VAdv-Ly0012-5mg 5 mg
EUR 2511
Description: Synthetic Monophosphoryl Lipid A, pure compound, a TLR4-based vaccine adjuvant.
Synthetic Monophosphoryl Lipid A
VAdv-Ly0031 1 mg
EUR 2131
Description: Synthetic monophosphoryl lipid A, a TLR4 agonist vaccine adjuvant.
Anti-Lipid A antibody
STJ16100142 1 mL
EUR 289
Goat Lipid peroxide ELISA kit
E06L0263-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Lipid peroxide ELISA kit
E06L0263-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Lipid peroxide ELISA kit
E06L0263-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Lipid peroxide ELISA kit
E02L0263-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Lipid peroxide ELISA kit
E02L0263-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Lipid peroxide ELISA kit
E02L0263-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Lipid peroxide ELISA kit
E03L0263-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.