Minute Detergent-Free Total Protein Extraction Kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly extract detergent-free total proteins from cultured cells (insect/mammalian/other cultured cells) and animal tissues.

The protein extraction buffers do not contain any detergent and EDTA. The extraction volume can be as small as 20 μl and as large as 500 μl with the use of the protein extraction filter cartridges. When the available starting material is a limiting factor, this can be quite useful. Detergent-free total proteins can be extracted from cultured cells/tissues in less than 5 minutes with a high yield (1-5 mg/ml).

 

Kit includes:

Items Quantity
Buffer A 15 ml
Buffer B 15 ml
Protein Extraction Filter Cartridges 50 units
Collection Tubes with Cap 50 units
Plastic Rods 2 units

 

The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).
• Extracts highly pure total RNA (including small RNA fraction) from exosomes
• Recovers protein and RNA from the same sample
• Compatible with all protocols for exosome isolation

The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function.

Detergent Free Exosomal Protein

Detergent Free Exosomal Protein

The kit allows the recovery of both RNA and proteins from the same purified exosome preparation. One portion of the sample is subjected to organic extraction, followed by immobilization of RNA on glass-fiber filters to purify total RNA or enrich for small RNA species. The other portion is then used directly for common protein applications such as Western blotting.

High yields of ultra-pure RNA can be prepared in about 30–60 minutes. The RNA can then be used in a variety of downstream applications, including qRT-PCR, high-throughput sequencing (e.g., using the Ion Torent PGM, Ion Torrent Proton, or SOLiD sequencing instruments), microarray analysis, solution hybridization assays, and blot hybridization.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

 

HYPERFLASK,CORNING,CELLBIND SURFACE,MANUAL,1720CM2,1/4

10030 1/pk
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ProPette MPC, Manual Pipette Controller with filter

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Description: Sealing Products; Sealing mats - Axygen
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