DESCRIPTION

mCherry is the second generation monomeric red fluorescent protein that was derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), such as GFP or DsRed. mCherry is widely used as a fluorescent tracer in transfection and transgenic experiments because of its better performance in brightness, photostability and monomeric structure. Since mCherry requires no additional substrates or cofactors, mCherry can be easily detected under a fluorescence microscope.

However, most imaging studies of mCherry are only qualitative. BioVision’s mCherry Quantification Kit provides an easy 96 micro-plate assay to analyze mCherry expression level in cells or tissues quantitatively. Cells or tissues can be homogenized directly in the mCherry Assay Buffer. The amount of mCherry is determined by comparing its fluorescence (Ex/Em= 587/610 nm) with a mCherry standard that is provided in this kit. Each kit provides sufficient reagents to perform up to 100 assays.

 

mCherry Quantification Kit

mCherry Quantification Kit

IHC using an anti-mCherry antibody (fluorescent and stained image). Intestinal tissue from a transgenic mouse expressing mCherry in all tissues. (A) Fluorescent image detecting mCherry expression and (B) HRP-stained image. Following isolation and fixation in 4% paraformaldehyde, mCherry Monoclonal Antibody (16D7) (Cat. No. M11217) was used at a 1:15,000 dilution. The ImmPRESS™ Anti-Rat Ig (peroxidase) Polymer Detection Kit (Vector Laboratories) was used according to manufacturer’s instructions; sections were incubated in peroxidase substrate solution until the desired stain intensity developed.

Flow cytometry analysis of cells expressing mCherry. U20S cells expressing mCherry were analyzed using 405 nm excitation and 450/40 nm band pass emission on an Attune Acoustic Focusing Cytometer. The histogram shows cells stained with mCherry rat monoclonal antibody conjugated with Pacific Blue (black line) and unstained cells (gray line).

Cat # +Size K971-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 587/610 nm)
Species Reactivity Mammalian
Applications It detects a wide range of mCherry (0.01-1 ng/µl)
Features & Benefits • Simple procedure; takes ~ 20 minutes
• Fast and convenient
Kit Components • mCherry Assay Buffer
• mCherry Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

 

mCherry Antibody (PA5-34974) in WB

Western Blot analysis of mCherry was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a mCherry Polyclonal Antibody (Product # PA5-34974) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

mCherry Antibody

5993-100
EUR 403

mCherry Antibody

5993-30T
EUR 146

mCherry Antibody

20-abx159607
  • EUR 272.00
  • EUR 230.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

mCherry Antibody

abx332840-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

mCherry

MO22192 100 ul
EUR 435

mCherry-Tag Antibody

1-CSB-PA000343
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Affinity purification
Description: A polyclonal antibody against mCherry-Tag. Recognizes mCherry-Tag from . This antibody is Unconjugated. Tested in the following application: WB;WB:1:5000

mCherry tag Antibody

abx019132-100ug 100 ug
EUR 356
  • Shipped within 5-10 working days.

mCherry-tag Antibody

20-abx134448
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

mCherry-tag Antibody

20-abx134449
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

mCherry-tag Antibody

20-abx134450
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

mCherry-Tag Antibody

20-abx242885
  • EUR 328.00
  • EUR 272.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

mCherry-Tag Antibody

20-abx330249
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

mCherry-Tag Antibody

20-abx330282
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

mCherry Monoclonal Antibody

ABM40125-003ml 0.03ml
EUR 158
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein

mCherry Monoclonal Antibody

ABM40125-01ml 0.1ml
EUR 289
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein

mCherry Monoclonal Antibody

ABM40125-02ml 0.2ml
EUR 414
  • Immunogen information: Recombinant Protein
  • Applications tips:
Description: A monoclonal antibody for detection of mCherry from Null. This mCherry antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein

Anti-mCherry antibody

STJ120297 100 µl
EUR 526

Anti-mCherry antibody

STJ140000 300 µg
EUR 270
Description: Goat polyclonal antibody to mCherry (Cherry fluorescent protein).

Anti-mCherry antibody

STJ140001 300 µg
EUR 270
Description: Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Anti-mCherry antibody

STJ97027 200 µl
EUR 197
Description: Mouse monoclonal to mCherry.

mCherry-Tag Antibody

T0090 1ml
EUR 920
Description: The mCherry tag antibody can recognize mCherry and mCherry-tag fusion proteins.

mCherry-Chicken

CH22115 100 ul
EUR 435

mCherry - Mouse

MO22140 100 ul
EUR 435

pET28a- mCherry

PVT0075 2 ug
EUR 256

pNanog- mCherry

PVT10426 2 ug
EUR 266
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