Primarily primarily most likely essentially the most normally used histological stain, hematoxylin is a pure compound extracted from a species of tree current in Mexico and the West Indies. The extracted compound is oxidized to offer hematein, the energetic staining ingredient of the hematoxylin stain.
The manufacturing of histological staining alternatives is an work. Just some laboratories are capable of making a stain of reliable vibrance. At Nationwide Diagnostics we’ve now now now gone one step extra.

Histo-Clear II

  • Low Hazard
  • Biodegradable
  • Lowered Citrus Odor

Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that ends in the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and fully biodegradable, thus decreasing disposal costs. Histo-Clear II has a vastly diminished citrus odor as in distinction with totally completely totally different citrus-based histological clearing brokers.

Uncover: Histo-Clear II is simply not relevant with many synthetic mounting media. Histo-Clear II is a long-standing favorite dry mounting procedures and aqueous mounting. Please affirm compatibility alongside collectively collectively together with your mounting media earlier than using Histo-Clear II with in wet-mounting with synthetic media.

 

Hazardous Provides Worth (HazMat) applies if shipped by Air.

PROCEDURE

Uncover: The next protocol has given good to good outcomes with every kind of tissue sections. It
would possibly ought to be optimized for a given tissue, half
thickness, and so forth.

National Diagnostics HISTO-CLEAR

Nationwide Diagnostics HISTO-CLEAR

1. Dewax and rehydrate slides

a) Immerse slides inside the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes

2. Stain
a) Immerse slides in Hematoxylin reply for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for 2 minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.

3. Dehydrate, clear and mount
a) Immerse slides inside the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.

 

Histo-Clear
NAT1332 5 US gallons
EUR 583
Histo-Clear II
NAT1334 1 US gallon
EUR 126
Histo-Clear II
NAT1336 5 US gallons
EUR 381
Calci-Clear
NAT1312 EACH
EUR 78
Calci-Clear
NAT1314 EACH
EUR 128
Calci-Clear
NAT1316 EACH
EUR 386
Calci-Clear Rapid
NAT1318 EACH
EUR 75
Calci-Clear Rapid
NAT1320 EACH
EUR 128
Calci-Clear Rapid
NAT1322 EACH
EUR 386
10X PBS MaxTag Histo (OKRA00043)
OKRA00043 100 ml
EUR 140
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Hematoxylin Counterstain MaxTag Histo (OKRA00050)
OKRA00050 15 ml
EUR 140
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:
Diogenes Kit
NAT1000 1KIT
EUR 225
Hydrogen Peroxide Assay Kit
NAT1002 1KIT
EUR 257
ProtoBlock System
NAT1004 1KIT
EUR 102
450ML PBS 10X
NAT1006 450ML
EUR 80
4L PBS 10X
NAT1008 4L
EUR 199
ProtoGlow ECL 200ml Kit
NAT1010 EACH
EUR 295
ProtoGlow ECL 500ml Kit
NAT1012 EACH
EUR 523
100G Acrylamide
NAT1014 100G
EUR 100
500G Acrylamide
NAT1016 500G
EUR 218
1KG Acrylamide
NAT1018 1KG
EUR 344
25G AquaPor LE
NAT1020 25G
EUR 110
100G AquaPor LE GTAC
NAT1022 100G
EUR 209
500G AquaPor LE GTAC
NAT1024 500G
EUR 732
25G AquaPor ES
NAT1026 25G
EUR 127
100G AquaPor ES
NAT1028 100G
EUR 292