Primarily primarily most likely essentially the most normally used histological stain, hematoxylin is a pure compound extracted from a species of tree current in Mexico and the West Indies. The extracted compound is oxidized to offer hematein, the energetic staining ingredient of the hematoxylin stain.
The manufacturing of histological staining alternatives is an work. Just some laboratories are capable of making a stain of reliable vibrance. At Nationwide Diagnostics we’ve now now now gone one step extra.

Histo-Clear II

  • Low Hazard
  • Biodegradable
  • Lowered Citrus Odor

Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that ends in the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and fully biodegradable, thus decreasing disposal costs. Histo-Clear II has a vastly diminished citrus odor as in distinction with totally completely totally different citrus-based histological clearing brokers.

Uncover: Histo-Clear II is simply not relevant with many synthetic mounting media. Histo-Clear II is a long-standing favorite dry mounting procedures and aqueous mounting. Please affirm compatibility alongside collectively collectively together with your mounting media earlier than using Histo-Clear II with in wet-mounting with synthetic media.

 

Hazardous Provides Worth (HazMat) applies if shipped by Air.

PROCEDURE

Uncover: The next protocol has given good to good outcomes with every kind of tissue sections. It
would possibly ought to be optimized for a given tissue, half
thickness, and so forth.

National Diagnostics HISTO-CLEAR

Nationwide Diagnostics HISTO-CLEAR

1. Dewax and rehydrate slides

a) Immerse slides inside the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes

2. Stain
a) Immerse slides in Hematoxylin reply for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for 2 minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.

3. Dehydrate, clear and mount
a) Immerse slides inside the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.

 

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