Precision

Intra-assay
Pattern n Degree out SD CV%
1 16 147pg/ml 10.29 = 7%
2 16 683pg/ml 49.85 = 7.3%
3 16 2750pg/ml 206.25 = 7.5%
Inter-assay
Pattern n Degree out SD CV%
1 24 156pg/ml 13.88 = 8.9%
2 24 700pg/ml 62.3 = 8.9%
3 24 2531pg/ml 227.05 = 8.9%

Product overview

The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom-made supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA gear relies upon on commonplace sandwich enzyme-linked immune-sorbent assay expertise. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Requirements (Expression system for frequent: NSO; Immunogen sequence: S7-A71) and try samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Delicate is added and unbound conjugates are washed away with PBS or TBS buffer.

Key alternatives and particulars

  • Sensitivity: 10 pg/ml
  • Differ: 78 pg/ml – 5000 pg/ml
  • Pattern sort: Cell custom-made supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection approach: Colorimetric
  • Assay sort: Sandwich (quantitative)
  • Reacts with: Human
  • Product prepare

    Human BAFF-R ELISA Gadgets
  • Detection approach

    Colorimetric
  • Pattern sort

    Cell custom-made supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay sort

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Differ

    78 pg/ml – 5000 pg/ml
  • Assay time

    3h 30m
  • Assay measurement

    A substantial amount of steps commonplace assay
  • Species reactivity

    Reacts with: Human
bio human baff r

bio human baff r

 

  • Notes

    BAFF-R (moreover usually known as Tumor necrosis draw back receptor superfamily member 13C or B-cell-activating draw back receptor)  is a protein that in of us is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating draw back (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a sort III transmembrane protein containing a single extracellular phenylalanine-rich area. It’s thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

  • Platform

    Pre-coated microplate (12 x Eight appropriately strips)

BAFF-R Antibody

3578R-100
EUR 316

BAFF-R Antibody

3578R-30T
EUR 146

BIO-X-ACT Short DNA Polymerase

BIO-21065 500 Units Ask for price

BIO-X-ACT Short Mix, 2x

BIO-25026 500 Reactions Ask for price

BAFF-R Polyclonal Antibody

3578-100
EUR 316

BAFF-R Blocking Peptide

3578RBP-50
EUR 153

Polyclonal BAFF-R Antibody

APR15125G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications:

Anti-BAFF-R (human) Monoclonal Antibody (11C1)

M02865 100ug
EUR 446
Description: Mouse Monoclonal BAFF-R (human) Antibody (11C1). Validated in Flow Cytometry, IHC and tested in Human.

Human B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Hu-48T 48T
EUR 498
  • Should the Human B-Cell Activating Factor (BAFF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Hu-96T 96T
EUR 647
  • Should the Human B-Cell Activating Factor (BAFF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.