Abstract Previously, we developed a single recombinant protein vaccine against plague composed of a fusion between the Fraction 1 (F1) capsular antigen and the V antigen. To determine whether the overall expression, solubility, and recovery of the F1 fusion protein Read more
Introduction The PhoP/PhoQ two-component signal transduction system is an important regulator of stress responses and virulence in most gram-negative bacteria, but the characterization of PhoP/PhoQ in Shigella has not been thoroughly investigated. In the present study, we found that deletion Read more
Properties Form: lyophilized powder Quality level: 200 Storage temperature: 2-8°C Product Name: Bordetella pertussis Recombinants Genetic information Bordetella pertussis Tohama I … ptxA(2665068) , ptxB(2665069) , ptxC(2665408) General description The product is not activated. If activation is required, see Kaslow, Read more
Abstract We have implemented a simple, inexpensive and rapid procedure for the validation and performance verification of pipettes mounted on automatic liquid handlers (ALH) as required for ISO 17025 accredited laboratories. A serial dilution of six or seven passages of Read more
Introduction NGeneBio (Seoul, Korea) introduced its next-generation sequencing (NGS)-based oncology/genetic disease kits and NGenePlex nCoV qRT-PCR kit against COVID-19 at AACC 2021. Dedicated to achieving better health through laboratory medicine, the American Association for Clinical Chemistry, AACC, brings together more Read more
1. Expected use The Novel Coronavirus (2019-nCoV) Nucleic Acid Diagnostic Kit (PCR-Fluorescence Probe) is a real-time RT PCR test intended for the qualitative detection of SARS-CoV-2 nucleic acid in nasopharyngeal swabs, oropharyngeal (throat) swabs, anterior nasal swabs, middle turbinate swabs, Read more
Invitrogen dCTP is prepared at a final concentration of 100 mM in highly purified water. 100 mM dCTP is suitable for use in a polymerase chain reaction (PCR), sequencing, stuffing reactions, nick translation, cDNA synthesis, and TdT tracking reactions. Invitrogen’s Read more
Description It allows the synthesis of cDNA fragments of up to 9 kbp. and include modified bases. It is active within 55°C – 60°C, allows complex RNA templates to be used and provides reaction specificity. It has RNase H activity Read more
Lipid peroxidation assay protocol summary: – add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min – swap to wells of microplate – analyze with microplate reader For greater sensitivity, Read more
Background Information Chromatin immunoprecipitation (ChIP) offers an advantageous system for locating out protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), Read more